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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AM05355PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM05355PU-N, RRID:AB_1615492
- Product name
- anti FAK1 / PTK2
- Antibody type
- Monoclonal
- Antigen
- Hybridoma produced by the fusion of splenocytes from mice immunized with cells.Recombinant fragment of human FAK expressed in E. coli and mouse myeloma.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 34Q36
- Vial size
- 0.1 mg
- Concentration
- Lot specific
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- HeLa cells (lane 1), mouse L929 cells (lane 2), and rat PC12 cells (lane 3) were resolved by SDS-PAGE and transferred to PVDF. The membranes were incubated with this FAK monoclonal antibody (clone 34Q36) at a concentration of 1 μg/mL for two hours at room temperature. After washing, the membranes were incubated with a goat F(abâ)2 anti-mouse IgG alkaline phosphatase conjugated antibody at a 1:2000 dilution. Bands were detected with CDP-substrate using the WesternStarTM method (Tropix) and Kodak BioMax film.
