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Learn500-P38B-100UG
antibody from Invitrogen Antibodies
Targeting: CCL4
Act-2, AT744.1, LAG1, MIP-1-beta, SCYA4
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
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- Product number
- 500-P38B-100UG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CCL4 Polyclonal Antibody, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: APMGSDPPTA CCFSYTARKL PHNFVVDYYE TSSLCSQPAV VFQTKRGKQV CADPSESWVQ EYVYDLELN.
- Concentration
- 0.1-1.0 mg/mL
Submitted references Generation and growth of CD28nullCD8+ memory T cells mediated by IL-15 and its induced cytokines.
Chiu WK, Fann M, Weng NP
Journal of immunology (Baltimore, Md. : 1950) 2006 Dec 1;177(11):7802-10
Journal of immunology (Baltimore, Md. : 1950) 2006 Dec 1;177(11):7802-10
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect Human MIP-1beta by Western Blot analysis CCL4 Polyclonal Antibody (Product # 500-P38B-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human MIP-1beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect Human MIP-1beta by Western Blot analysis CCL4 Polyclonal Antibody (Product # 500-P38B-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human MIP-1beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA: To detect Human MIP-1beta (CCL4) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of CCL4 Polyclonal Antibody (Product # 500-P38B-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech CCL4 Polyclonal Antibody, Biotin (Product # 500-P38BBT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human MIP-1beta (CCL4).
