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- Antibody Data
- Antigen structure
- References [10]
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- Immunocytochemistry [1]
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- Blocking/Neutralizing [1]
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- Product number
- AF-271-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human CCL4/MIP-1 beta Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human CCL4/MIP-1 beta in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse (rm) MIP-1 alpha is observed, approximately 20% cross-reactivity with recombinant human (rh) MIP-1 alpha and recombinant cotton rat MIP-1 alpha is observed, and less than 1% cross-reactivity with rhMCP-2, rmMIP-1 beta , rmMCP-2, and recombinant viral MIP-1 beta is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Evaluation of Immunodiagnostic Tests for Leprosy in Brazil, China and Ethiopia.
An initial investigation into endothelial CC chemokine expression in the human rheumatoid synovium.
Increased Levels of Macrophage Inflammatory Proteins Result in Resistance to R5-Tropic HIV-1 in a Subset of Elite Controllers.
NK cell responses to simian immunodeficiency virus vaginal exposure in naive and vaccinated rhesus macaques.
A novel p38-MAPK signaling axis modulates neutrophil biology in head and neck cancer.
Villitis of unknown etiology is associated with a distinct pattern of chemokine up-regulation in the feto-maternal and placental compartments: implications for conjoint maternal allograft rejection and maternal anti-fetal graft-versus-host disease.
Intestinal neuro-epithelial interactions modulate neuronal chemokines production.
The beta chemokines CCL4 and CCL5 enhance adhesion of specific CD4+ T cell subsets to human brain endothelial cells.
The beta chemokines CCL4 and CCL5 enhance adhesion of specific CD4+ T cell subsets to human brain endothelial cells.
Simian immunodeficiency viruses with defective nef genes show increased susceptibility to the noncytotoxic antiviral activity of CD8+ lymphocytes.
van Hooij A, Tjon Kon Fat EM, Batista da Silva M, Carvalho Bouth R, Cunha Messias AC, Gobbo AR, Lema T, Bobosha K, Li J, Weng X, Salgado CG, Spencer JS, Corstjens PLAM, Geluk A
Scientific reports 2018 Dec 18;8(1):17920
Scientific reports 2018 Dec 18;8(1):17920
An initial investigation into endothelial CC chemokine expression in the human rheumatoid synovium.
Rump L, Mattey DL, Kehoe O, Middleton J
Cytokine 2017 Sep;97:133-140
Cytokine 2017 Sep;97:133-140
Increased Levels of Macrophage Inflammatory Proteins Result in Resistance to R5-Tropic HIV-1 in a Subset of Elite Controllers.
Walker WE, Kurscheid S, Joshi S, Lopez CA, Goh G, Choi M, Barakat L, Francis J, Fisher A, Kozal M, Zapata H, Shaw A, Lifton R, Sutton RE, Fikrig E
Journal of virology 2015 May;89(10):5502-14
Journal of virology 2015 May;89(10):5502-14
NK cell responses to simian immunodeficiency virus vaginal exposure in naive and vaccinated rhesus macaques.
Shang L, Smith AJ, Duan L, Perkey KE, Qu L, Wietgrefe S, Zupancic M, Southern PJ, Masek-Hammerman K, Reeves RK, Johnson RP, Haase AT
Journal of immunology (Baltimore, Md. : 1950) 2014 Jul 1;193(1):277-84
Journal of immunology (Baltimore, Md. : 1950) 2014 Jul 1;193(1):277-84
A novel p38-MAPK signaling axis modulates neutrophil biology in head and neck cancer.
Dumitru CA, Fechner MK, Hoffmann TK, Lang S, Brandau S
Journal of leukocyte biology 2012 Apr;91(4):591-8
Journal of leukocyte biology 2012 Apr;91(4):591-8
Villitis of unknown etiology is associated with a distinct pattern of chemokine up-regulation in the feto-maternal and placental compartments: implications for conjoint maternal allograft rejection and maternal anti-fetal graft-versus-host disease.
Kim MJ, Romero R, Kim CJ, Tarca AL, Chhauy S, LaJeunesse C, Lee DC, Draghici S, Gotsch F, Kusanovic JP, Hassan SS, Kim JS
Journal of immunology (Baltimore, Md. : 1950) 2009 Mar 15;182(6):3919-27
Journal of immunology (Baltimore, Md. : 1950) 2009 Mar 15;182(6):3919-27
Intestinal neuro-epithelial interactions modulate neuronal chemokines production.
Tixier E, Galmiche JP, Neunlist M
Biochemical and biophysical research communications 2006 Jun 2;344(2):554-61
Biochemical and biophysical research communications 2006 Jun 2;344(2):554-61
The beta chemokines CCL4 and CCL5 enhance adhesion of specific CD4+ T cell subsets to human brain endothelial cells.
Quandt J, Dorovini-Zis K
Journal of neuropathology and experimental neurology 2004 Apr;63(4):350-62
Journal of neuropathology and experimental neurology 2004 Apr;63(4):350-62
The beta chemokines CCL4 and CCL5 enhance adhesion of specific CD4+ T cell subsets to human brain endothelial cells.
Quandt J, Dorovini-Zis K
Journal of neuropathology and experimental neurology 2004 Apr;63(4):350-62
Journal of neuropathology and experimental neurology 2004 Apr;63(4):350-62
Simian immunodeficiency viruses with defective nef genes show increased susceptibility to the noncytotoxic antiviral activity of CD8+ lymphocytes.
Binninger-Schinzel D, Norley S, Adler HS, Oberg HH, Kurth R
Virology 2002 Mar 1;294(1):209-21
Virology 2002 Mar 1;294(1):209-21
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CCL4/MIP-1 beta in Human PBMCs. CCL4/MIP-1 beta was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PHA and monensin using Goat Anti-Human CCL4/MIP-1 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-271-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CCL4/MIP-1 beta in Human Alzheimer's Disease Brain. CCL4/MIP-1 beta was detected in immersion fixed paraffin-embedded sections of human Alzheimer's disease brain (hippocampus) using 15 µg/mL Goat Anti-Human CCL4/MIP-1 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-271-NA) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CCL4/MIP-1 beta and Neutral-ization by Human CCL4/MIP-1 beta Antibody. Recombinant Human CCL4/MIP-1 beta (Catalog # 271-BME) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL4/MIP-1 beta (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL4/MIP-1 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-271-NA). The ND50 is typically 0.3-1.5 µg/mL.
