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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- EB11532 - Provider product page
- Provider
- Everest Biotech
- Product name
- Goat anti-Mindbomb 1 / MIB1 Antibody
- Antibody type
- Polyclonal
- Description
- Goat anti-Mindbomb 1 / MIB1 Antibody
- Reactivity
- Human
- Host
- Goat
- Antigen sequence
C-DKDNTNVNADVQKLQ- Epitope
- internal region (near C Terminus)
- Isotype
- IgG
- Vial size
- 100µg specific antibody in 200µl
- Concentration
- 0.5 mg/ml
- Storage
- Aliquot and store at -20°C. Minimize freezing and thawing.
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Supportive validation
- Submitted by
- Everest Biotech (provider)
- Main image
- Experimental details
- HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human MIB1 with C-terminal MYC tag probed with EB11532 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with EB11532 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Everest Biotech (provider)
- Main image
- Experimental details
- EB11532 (2µg/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
