Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NB100-58968 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-58968, RRID:AB_877545
- Product name
- Goat Polyclonal SHP-1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognize both reported isoforms (NP_536858.1and NP_002822.2). Please note this antibody was designed using the mouse sequence, which differs by 1 amino acid from the human sequence.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Antigen sequence
KASRTSSKHKEE
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references The SHP-1 protein tyrosine phosphatase negatively modulates glucose homeostasis.
Dubois MJ, Bergeron S, Kim HJ, Dombrowski L, Perreault M, Fournès B, Faure R, Olivier M, Beauchemin N, Shulman GI, Siminovitch KA, Kim JK, Marette A
Nature medicine 2006 May;12(5):549-56
Nature medicine 2006 May;12(5):549-56
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SHP-1 Antibody [NB100-58968] - Staining of Human Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SHP-1 Antibody [NB100-58968] - Staining of Mouse Thymus lysate (A) with antibody at 0.01 ug/mL and Rat Thymus lysate with antibody at 0.1 ug/mL (B). 35 ug protein in RIPA buffer. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SHP-1 Antibody [NB100-58968] - Staining of paraffin embedded Human Thyroid. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.