H00026574-M04

antibody from Abnova Corporation

Targeting: AATF BFR2, CHE-1, CHE1, DED

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00026574-M04

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00026574-M04, RRID:AB_913233

Product name

AATF monoclonal antibody (M04), clone 3C7

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant AATF.

Antigen sequence

VQSISDFEKFTKGMDDLGSSEEEEDEESGMEEGDD
AEDSQGESEEDRAGDRNSEDDGVVMTFSSVKVSEE
VEKGRAVKNQIALWDQLLEGRIKLQKALLT


Isotype

IgG

Antibody clone number

3C7

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

AATF monoclonal antibody (M04), clone 3C7 Western Blot analysis of AATF expression in Hela S3 NE ( Cat # L013V3 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged AATF is 0.03 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to AATF on HeLa cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to AATF on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol