Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00338382-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00338382-M01, RRID:AB_547531
- Product name
- Mouse Monoclonal RAB7B Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. RAB7B - RAB7B, member RAS oncogene family
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Rab7a regulates cell migration through Rac1 and vimentin.
A novel interaction between Rab7b and actomyosin reveals a dual role in intracellular transport and cell migration.
Clathrin-dependent mechanisms modulate the subcellular distribution of class C Vps/HOPS tether subunits in polarized and nonpolarized cells.
GIGYF2 is present in endosomal compartments in the mammalian brains and enhances IGF-1-induced ERK1/2 activation.
Abnormal localization of leucine-rich repeat kinase 2 to the endosomal-lysosomal compartment in lewy body disease.
SPE-39 family proteins interact with the HOPS complex and function in lysosomal delivery.
Margiotta A, Progida C, Bakke O, Bucci C
Biochimica et biophysica acta. Molecular cell research 2017 Feb;1864(2):367-381
Biochimica et biophysica acta. Molecular cell research 2017 Feb;1864(2):367-381
A novel interaction between Rab7b and actomyosin reveals a dual role in intracellular transport and cell migration.
Borg M, Bakke O, Progida C
Journal of cell science 2014 Nov 15;127(Pt 22):4927-39
Journal of cell science 2014 Nov 15;127(Pt 22):4927-39
Clathrin-dependent mechanisms modulate the subcellular distribution of class C Vps/HOPS tether subunits in polarized and nonpolarized cells.
Zlatic SA, Tornieri K, L'Hernault SW, Faundez V
Molecular biology of the cell 2011 May 15;22(10):1699-715
Molecular biology of the cell 2011 May 15;22(10):1699-715
GIGYF2 is present in endosomal compartments in the mammalian brains and enhances IGF-1-induced ERK1/2 activation.
Higashi S, Iseki E, Minegishi M, Togo T, Kabuta T, Wada K
Journal of neurochemistry 2010 Oct;115(2):423-37
Journal of neurochemistry 2010 Oct;115(2):423-37
Abnormal localization of leucine-rich repeat kinase 2 to the endosomal-lysosomal compartment in lewy body disease.
Higashi S, Moore DJ, Yamamoto R, Minegishi M, Sato K, Togo T, Katsuse O, Uchikado H, Furukawa Y, Hino H, Kosaka K, Emson PC, Wada K, Dawson VL, Dawson TM, Arai H, Iseki E
Journal of neuropathology and experimental neurology 2009 Sep;68(9):994-1005
Journal of neuropathology and experimental neurology 2009 Sep;68(9):994-1005
SPE-39 family proteins interact with the HOPS complex and function in lysosomal delivery.
Zhu GD, Salazar G, Zlatic SA, Fiza B, Doucette MM, Heilman CJ, Levey AI, Faundez V, L'hernault SW
Molecular biology of the cell 2009 Feb;20(4):1223-40
Molecular biology of the cell 2009 Feb;20(4):1223-40
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RAB7B Antibody (3B3) [H00338382-M01] - RAB7B expression in HeLa ( Cat # L013V1 ).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RAB7B Antibody (3B3) [H00338382-M01] - Analysis of RAB7B expression in transfected 293T cell line by RAB7B monoclonal antibody (M01), clone 3B3.Lane 1: RAB7B transfected lysate(22.5 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RAB7B Antibody (3B3) [H00338382-M01] - RAB7B monoclonal antibody (M01), clone 3B3. Analysis of RAB7B expression in A-431.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: RAB7B Antibody (3B3) [H00338382-M01] - Detection limit for recombinant GST tagged RAB7B is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: RAB7B Antibody (3B3) [H00338382-M01] - Monoclonal antibody to RAB7B (H00338382-M01) on HeLa cell . [antibody concentration 10 ug/ml]
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: RAB7B Antibody (3B3) [H00338382-M01] - Mouse primary cells stained with RAB7B antibody. Image from verified customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: RAB7B Antibody (3B3) [H00338382-M01] - Analysis of RAB7B transfected lysate using anti-RAB7B monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RAB7B MaxPab rabbit polyclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RAB7B Antibody (3B3) [H00338382-M01] - Monoclonal antibody to RAB7B ( Cat # H00338382-M01 ) on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]