AF2535

antibody from R&D Systems

Targeting: MRC1 bA541I19.1, CD206, CLEC13D, CLEC13DL, MRC1L1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

AF2535

Provider

R&D Systems

Product name

Mouse MMR/CD206 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects mouse MMR/CD206 in direct ELISAs and Western blots. In direct ELISAs, less than 45% cross-reactivity with recombinant human MMR is observed.

Reactivity

Mouse

Host

Goat

Conjugate

Unconjugated

Antigen sequence

Q2HZ94


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Microglia are an essential component of the neuroprotective scar that forms after spinal cord injury.

Bellver-Landete V, Bretheau F, Mailhot B, Vallières N, Lessard M, Janelle ME, Vernoux N, Tremblay MÈ, Fuehrmann T, Shoichet MS, Lacroix S
Nature communications 2019 Jan 31;10(1):518

---

Responses of perivascular macrophages to circulating lipopolysaccharides in the subfornical organ with special reference to endotoxin tolerance.

Morita-Takemura S, Nakahara K, Hasegawa-Ishii S, Isonishi A, Tatsumi K, Okuda H, Tanaka T, Kitabatake M, Ito T, Wanaka A
Journal of neuroinflammation 2019 Feb 14;16(1):39

---

Transiently proliferating perivascular microglia harbor M1 type and precede cerebrovascular changes in a chronic hypertension model.

Koizumi T, Taguchi K, Mizuta I, Toba H, Ohigashi M, Onishi O, Ikoma K, Miyata S, Nakata T, Tanaka M, Foulquier S, Steinbusch HWM, Mizuno T
Journal of neuroinflammation 2019 Apr 10;16(1):79

---

Microglial TLR4-dependent autophagy induces ischemic white matter damage via STAT1/6 pathway.

Qin C, Liu Q, Hu ZW, Zhou LQ, Shang K, Bosco DB, Wu LJ, Tian DS, Wang W
Theranostics 2018;8(19):5434-5451

---

Interleukin-12p35 Knock Out Aggravates Doxorubicin-Induced Cardiac Injury and Dysfunction by Aggravating the Inflammatory Response, Oxidative Stress, Apoptosis and Autophagy in Mice.

Ye J, Huang Y, Que B, Chang C, Liu W, Hu H, Liu L, Shi Y, Wang Y, Wang M, Zeng T, Zhen W, Xu Y, Shi L, Liu J, Jiang H, Ye D, Lin Y, Wan J, Ji Q
EBioMedicine 2018 Sep;35:29-39

---

2-carba cyclic phosphatidic acid suppresses inflammation via regulation of microglial polarisation in the stab-wounded mouse cerebral cortex.

Hashimoto K, Nakashima M, Hamano A, Gotoh M, Ikeshima-Kataoka H, Murakami-Murofushi K, Miyamoto Y
Scientific reports 2018 Jun 26;8(1):9715

---

Molecular and cellular identification of the immune response in peripheral ganglia following nerve injury.

Lindborg JA, Niemi JP, Howarth MA, Liu KW, Moore CZ, Mahajan D, Zigmond RE
Journal of neuroinflammation 2018 Jun 26;15(1):192

---

B-cell leukemia/lymphoma 10 promotes angiogenesis in an experimental corneal neovascularization model.

Liu G, Lu P, Chen L, Zhang W, Wang M, Li D, Zhang X
Eye (London, England) 2018 Jul;32(7):1220-1231

---

Serelaxin induces Notch1 signaling and alleviates hepatocellular damage in orthotopic liver transplantation.

Kageyama S, Nakamura K, Ke B, Busuttil RW, Kupiec-Weglinski JW
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2018 Jul;18(7):1755-1763

---

Salidroside provides neuroprotection by modulating microglial polarization after cerebral ischemia.

Liu X, Wen S, Yan F, Liu K, Liu L, Wang L, Zhao S, Ji X
Journal of neuroinflammation 2018 Feb 9;15(1):39

---

ST2/IL-33-Dependent Microglial Response Limits Acute Ischemic Brain Injury.

Yang Y, Liu H, Zhang H, Ye Q, Wang J, Yang B, Mao L, Zhu W, Leak RK, Xiao B, Lu B, Chen J, Hu X
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 May 3;37(18):4692-4704

---

Predictive screening of M1 and M2 macrophages reveals the immunomodulatory effectiveness of post spinal cord injury azithromycin treatment.

Gensel JC, Kopper TJ, Zhang B, Orr MB, Bailey WM
Scientific reports 2017 Jan 6;7:40144

---

Targeting mannose receptor expression on macrophages in atherosclerotic plaques of apolipoprotein E-knockout mice using ¹¹¹In-tilmanocept.

Varasteh Z, Hyafil F, Anizan N, Diallo D, Aid-Launais R, Mohanta S, Li Y, Braeuer M, Steiger K, Vigne J, Qin Z, Nekolla SG, Fabre JE, Döring Y, Le Guludec D, Habenicht A, Vera DR, Schwaiger M
EJNMMI research 2017 Dec;7(1):40

---

HDAC3 inhibition ameliorates spinal cord injury by immunomodulation.

Kuboyama T, Wahane S, Huang Y, Zhou X, Wong JK, Koemeter-Cox A, Martini M, Friedel RH, Zou H
Scientific reports 2017 Aug 17;7(1):8641

---

Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice.

Sung SJ, Ge Y, Dai C, Wang H, Fu SM, Sharma R, Hahn YS, Yu J, Le TH, Okusa MD, Bolton WK, Lawler JR
Journal of immunology (Baltimore, Md. : 1950) 2017 Apr 1;198(7):2589-2601

---

Temporal Characterization of Microglia/Macrophage Phenotypes in a Mouse Model of Neonatal Hypoxic-Ischemic Brain Injury.

Hellström Erkenstam N, Smith PL, Fleiss B, Nair S, Svedin P, Wang W, Boström M, Gressens P, Hagberg H, Brown KL, Sävman K, Mallard C
Frontiers in cellular neuroscience 2016;10:286

---

Rescue therapy with Tanshinone IIA hinders transition of acute kidney injury to chronic kidney disease via targeting GSK3β.

Jiang C, Zhu W, Yan X, Shao Q, Xu B, Zhang M, Gong R
Scientific reports 2016 Nov 18;6:36698

---

miR-155 Deletion in Mice Overcomes Neuron-Intrinsic and Neuron-Extrinsic Barriers to Spinal Cord Repair.

Gaudet AD, Mandrekar-Colucci S, Hall JC, Sweet DR, Schmitt PJ, Xu X, Guan Z, Mo X, Guerau-de-Arellano M, Popovich PG
The Journal of neuroscience : the official journal of the Society for Neuroscience 2016 Aug 10;36(32):8516-32

---

Arginine deprivation and immune suppression in a mouse model of Alzheimer's disease.

Kan MJ, Lee JE, Wilson JG, Everhart AL, Brown CM, Hoofnagle AN, Jansen M, Vitek MP, Gunn MD, Colton CA
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Apr 15;35(15):5969-82

---

Galectin-1-secreting neural stem cells elicit long-term neuroprotection against ischemic brain injury.

Wang J, Xia J, Zhang F, Shi Y, Wu Y, Pu H, Liou AK, Leak RK, Yu X, Chen L, Chen J
Scientific reports 2015 Apr 10;5:9621

---

Expression of the homeobox gene HOXA9 in ovarian cancer induces peritoneal macrophages to acquire an M2 tumor-promoting phenotype.

Ko SY, Ladanyi A, Lengyel E, Naora H
The American journal of pathology 2014 Jan;184(1):271-81

---

Heterogeneous induction of microglia M2a phenotype by central administration of interleukin-4.

Pepe G, Calderazzi G, De Maglie M, Villa AM, Vegeto E
Journal of neuroinflammation 2014 Dec 31;11:211

---

MicroRNA-124 as a novel treatment for persistent hyperalgesia.

Willemen HL, Huo XJ, Mao-Ying QL, Zijlstra J, Heijnen CJ, Kavelaars A
Journal of neuroinflammation 2012 Jun 25;9:143

---

p47phox deficiency induces macrophage dysfunction resulting in progressive crystalline macrophage pneumonia.

Liu Q, Cheng LI, Yi L, Zhu N, Wood A, Changpriroa CM, Ward JM, Jackson SH
The American journal of pathology 2009 Jan;174(1):153-63

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Mouse MMR/CD206 by Western Blot. Western blot shows lysates of mouse liver tissue. Gels were loaded with 12 µg, 6.5 µg, and 3 µg of tissue lysate. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2535) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MMR/CD206 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

MMR/CD206 in Mouse Testis. MMR/CD206 was detected in perfusion fixed frozen sections of mouse testis using Goat Anti-Mouse MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2535) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to spermatocytes in testis. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

MMR/CD206 in Mouse Lung. MMR/CD206 was detected in perfusion fixed frozen sections of mouse lung using Goat Anti-Mouse MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2535) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm of macrophages. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.