Explore
Validate
LearnPA5-101657
antibody from Invitrogen Antibodies
Targeting: MRC1
bA541I19.1, CD206, CLEC13D, CLEC13DL, MRC1L1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-101657 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD206 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Real-time visualization of phagosomal pH manipulation by Cryptococcus neoformans in an immune signal-dependent way.
Deficiency of ITGAM Attenuates Experimental Abdominal Aortic Aneurysm in Mice.
Santiago-Burgos EJ, Stuckey PV, Santiago-Tirado FH
Frontiers in cellular and infection microbiology 2022;12:967486
Frontiers in cellular and infection microbiology 2022;12:967486
Deficiency of ITGAM Attenuates Experimental Abdominal Aortic Aneurysm in Mice.
Zhou M, Wang X, Shi Y, Ding Y, Li X, Xie T, Shi Z, Fu W
Journal of the American Heart Association 2021 Apr 6;10(7):e019900
Journal of the American Heart Association 2021 Apr 6;10(7):e019900
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CD206 in myeloma cells (Lane 1: treated with the antigen-specific peptide). Samples were incubated with CD206 polyclonal antibody (Product # PA5-101657).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CD206 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and CD206 polyclonal antibody (Product # PA5-101657) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of paraffin-embedded CD206 in mouse lung tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with CD206 polyclonal antibody (Product # PA5-101657) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 ITGAM deficiency ameliorates macrophage infiltration and polarization. A , Representative images of immunohistochemical staining for F4/80, MMP2, and MMP9 in the aortic tissues of saline- or CaCl 2 -treated WT or ITGAM(-/-) mice. Scale bar=100 mum. B , Quantification of F4/80-positive macrophages in the infrarenal abdominal aortas of the mice from the 3 experimental groups under a microscope (n=6 for each group), ** P
