PAB28493

antibody from Abnova Corporation

Targeting: INTS6 DBI-1, DDX26, DDX26A, DICE1, HDB, INT6, Notchl2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28493

Provider

Abnova Corporation

Product name

INTS6 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant INTS6

Antigen sequence

VVNNHIGGKGPPAPTTQAQPDLIKPLPLHKISETT
NDSIIHDVVENHVADQLSSDITPNAMDTEFSASSP
ASLLERPTNHMEALGHDHLGTNDLTVGGFLENHEE
PRDKEQCAEENIPASSLNKGKKLMHCRSHEEVNTE
LKAQ


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: A-431, Lane 4: Liver, Lane 5: Tonsil with INTS6 polyclonal antibody at 1:250 - 1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251MG with INTS6 polyclonal antibody (Cat # PAB28493) at 1-4 ug/ml shows positivity in nucleus but not nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human testis with INTS6 polyclonal antibody (Cat # PAB28493) shows cytoplasmic positivity in trophoblastic cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)