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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- STJ98549 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-SMAD5 antibody (C-Term) (STJ98549)
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody anti-Mothers Against Decapentaplegic Homolog 5 (C-Term) is suitable for use in Western Blot, Immunofluorescence, Immunocytochemistry and Flow Cytometry research applications.
- Reactivity
- Human, Mouse, Rat, Bovine, Chicken/Avian, Porcine, Sheep
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
NA- Epitope
- NA
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
- Handling
- NA
No comments: Submit comment
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Western blot analysis using Smad5 monoclonal antibody against mouse testis lysate.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using Smad5 monoclonal antibody.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells stained with Smad5 monoclonal antibody (red) , followed by FITC-conjugated goat anti-mouse IgG. Black line histogram represents the isotype control, normal mouse IgG.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
