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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- ARG55249 - Provider product page
- Provider
- Arigo
- Product name
- anti-DUX4 antibody [P4H2]
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide around 76 aa (C-terminus) of Human DUX4 with GST tag.
- Description
- Purification with Protein G.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- P4H2
- Vial size
- 50 µg
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to 1-2 weeks. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: C2C12 cells transfected with pCS2-DUX4 stained with ARG55249 anti-DUX4 antibody [P4H2] at 1:10000 dilution. There is a doublet because the expression construct contains an upstream alternate translation start codon. The lower band is the canonical size for DUX4.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: Rhabdomyosarcoma cell line RD transfected with pCS2-DUX4, stained with ARG55249 anti-DUX4 antibody [P4H2] (green) at 1:10000 dilution. Cells were counterstained with DAPI (blue) for nuclei.
