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Learn14315-1-AP
antibody from Invitrogen Antibodies
Targeting: SLC38A3
G17, SN1
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 14315-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SLC38A3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MEAPLQTEM VELVPNGKHS EGLLPVITPM AGNQRVEDPA RSCMEGKSFL QKSPSKEPHF TDFEGKTSFG MSVFNLSNAI MGSGILGLAY AMANTGIILF LFLLTAVALL SSYSIHLLLK SSGVVGIRAY EQLGYRAFGT PGKLAAALAI TLQNIGAMSS YLYIIKSELP LVIQTFLNLE EKTSDWYMNG NY (1-191 aa encoded by BC042875)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.27 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 14315-1-AP (SLC38A3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human heart tissue were subjected to SDS PAGE followed by western blot with 14315-1-AP (SLC38A3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 14315-1-AP (SLC38A3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BGC-823 cells were subjected to SDS PAGE followed by western blot with 14315-1-AP (SLC38A3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol) fixed HEK-293 cells using 14315-1-AP (SLC38A3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer using 14315-1-AP (SLC38A3 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer using 14315-1-AP (SLC38A3 antibody) at dilution of 1:100 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HeLa cells were stained with 0.2ug SLC38A3 antibody (14315-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-SLC38A3 (IP:14315-1-AP, 3ug; Detection:14315-1-AP 1:1000) with mouse heart tissue lysate 4000ug.
