GTX12862

antibody from GeneTex

Targeting: SYK

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

GTX12862

Provider

GeneTex

Proper citation

GeneTex Cat#GTX12862, RRID:AB_373050

Product name

Syk (phospho Tyr323) antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Extracts of Jurkat cells untreated (1) or treated with 10 mM H2O2 for 3 minutes (2-6) were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was either left untreated (1-5) or treated with YOP phosphatase (6), then blocked with a 3% milk-TBST buffer for one hour at room temperature, and incubated with the Syk [pY323] antibody for two hours at room temperature in a 3% milk-TBST buffer, following prior incubation with: no peptide (1, 2, 6), the non-phosphopeptide corresponding to the phosphopeptide immunogen (3), a generic phosphotyrosine-containing peptide (4), or the phosphopeptide immunogen (5). After washing, the membrane was incubated with goat F(ab¡¦)2 anti-rabbit IgG HRP conjugate and signals were detected. The data show that only the phosphopeptide corresponding to Syk [pY323] blocks the antibody signal, demonstrating the specificity of the antibody. The data also show the up-regulation of phosphorylation upon treatment with H2O2 in this cell system and that phosphatase stripping eliminates the signal, further verifying that the antibody is phospho-specific.