Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 44-233G - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-Syk (Tyr317) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20°C
Submitted references An Allosteric Shift in CD11c Affinity Activates a Proatherogenic State in Arrested Intermediate Monocytes.
Hernandez AA, Foster GA, Soderberg SR, Fernandez A, Reynolds MB, Orser MK, Bailey KA, Rogers JH, Singh GD, Wu H, Passerini AG, Simon SI
Journal of immunology (Baltimore, Md. : 1950) 2020 Nov 15;205(10):2806-2820
Journal of immunology (Baltimore, Md. : 1950) 2020 Nov 15;205(10):2806-2820
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Peptide Competition and Phosphatase Treatment. Lysates prepared from Jurkat cells left unstimulated (1) or stimulated with H2O2 (2-6) and mouse OKT-5 lymphocytes left unstimulated (7) or stimulated with H2O2 (8) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (Lanes 1-5, 7 & 8) or treated with L-phosphatase (6), blocked with a 3% Milk-TBST buffer for one hour at room temperature, and incubated with Syk (pY317) antibody for two hours at room temperature in a 3% Milk-TBST buffer, following prior incubation with: no peptide (1, 2, 6), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphotyrosine-containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F (ab’)2 anti-rabbit IgG HRP conjugate (Product # ALI4404) and bands were detected using the Pierce SuperSignal™ method. The data show that the signal was induced upon H2O2 treatment. The data also show peptide corresponding to Syk (pY317) blocks the antibody signal, and that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Peptide Competition and Phosphatase Treatment. Lysates prepared from Jurkat cells left unstimulated (1) or stimulated with H2O2 (2-6) and mouse OKT-5 lymphocytes left unstimulated (7) or stimulated with H2O2 (8) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (Lanes 1-5, 7 & 8) or treated with L-phosphatase (6), blocked with a 3% Milk-TBST buffer for one hour at room temperature, and incubated with Syk (pY317) antibody for two hours at room temperature in a 3% Milk-TBST buffer, following prior incubation with: no peptide (1, 2, 6), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphotyrosine-containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F (ab’)2 anti-rabbit IgG HRP conjugate (Product # ALI4404) and bands were detected using the Pierce SuperSignal™ method. The data show that the signal was induced upon H2O2 treatment. The data also show peptide corresponding to Syk (pY317) blocks the antibody signal, and that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.