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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- MA5-17207 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Syk Monoclonal Antibody (12E3)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control for this product is THP-1 cells.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 12E3
- Vial size
- 100 µL
- Storage
- -20°C
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of Ramos (Lane 1), THP-1 (Lane 2) and Jurkat (Lane 3). The blot was probed with Anti-Syk Monoclonal Antibody (Product # MA5-17207, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 72 kDa band corresponding to Syk was observed in relevant cell lines.
