Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-36692 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-Syk (Tyr525) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 72 kDa. Purity is >95% by SDS-PAGE.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-Syk (Tyr525) in Lane 1: HEK293T whole cell lysate (40 µg), Lane 2: HEK293T treated with UV for 5 minutes then repair for 4 hours whole cell lysate (40 µg), Lane 3: HEK293T treated with UV for 5 minutes then repair for 6 hours whole cell lysate (40 µg), Lane 4: HEK293T treated with UV for 5 minutes then repair for 16 hours whole cell lysate (40 µg), Lane 5: HEK293T treated with UV for 5 minutes then repair for 24 hours whole cell lysate (40 µg), Lane 6: the Lung tissue lysate of rat (40 µg), Lane 7: the Uterus tissue lysate of mouse (40 µg). Samples were incubated with Phospho-Syk (Tyr525) polyclonal antibody (Product # PA5-36692) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-Syk pTyr525 in paraffin-embedded human breast carcinoma using Phospho-Syk pTyr525 polyclonal antibody (Product # PA5-36692) at a dilution of 1:100.