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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-28016 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-MMP28 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- PA5-28016 targets MMP28 in IF and WB applications and shows reactivity with Human samples. The PA5-28016 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 335 of Human MMP28. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.63 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MMP28 using 30 µg Rat2 whole cell lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a MMP28 polyclonal antibody (Product # PA5-28016) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MMP28 using 30 µg of H1299 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a MMP28 polyclonal antibody (Product # PA5-28016) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of MMP28 in the cytosol of paraffin-embedded colon carcinoma using a MMP28 polyclonal antibody (Product # PA5-28016) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MMP28 in methanol-fixed HeLa cells using a MMP28 polyclonal antibody (Product # PA5-28016) at a 1:200 dilution.
