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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 844903 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#844903, RRID:AB_2566003
- Product name
- Ultra-LEAF™ Purified anti-Histone Lysine Demethylase (NO66)
- Antibody type
- Monoclonal
- Antigen
- Fusion protein consisting of NO66 protein fragment (aa 423-427) and Hepatitis B virus capsid protein.
- Description
- Histone Lysine Demethylase (NO66) is an oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. It specfically demethylates 'Lys-4' (H3K4me) and 'Lys-36' (H3K36me) of histone H3, thereby playing a central role in the histone code.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 8A7-G10
- Vial size
- 100 µg
- Concentration
- 1.0 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- 70 µg of whole cell protein were separated by SDS-PAGE, transferred to a nitrocellulose membrane, and probed with αNO66 (clone 8A7-G10) diluted 1:100. The exposure time was 30 seconds.
- Conjugate
- Unconjugated
