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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- TA328752 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal Anti-Orai1 (extracellular)
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal Anti-Orai1 (extracellular)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- Orai1
- Antibody clone number
- NULL
- Vial size
- 200 µl
- Concentration
- NULL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of rat spleen (lanes 1 and4), rat pancreas (lanes 2 and 5) and mouse B-cell lymphoma (WEHI) (lanes 3 and 6) lysates: 1-3. Anti-Orai1 (extracellular) antibody, (1:200). 4-6. Anti-Orai1 (extracellular) antibody, preincubated with the control peptide antigen.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Expression of Orai1 in rat RBL cells.Immunocytochemical staining of intact living rat basophilic leukemia (RBL) cells. A. Extracellular staining of cells using Anti-Orai1 (extracellular) antibody, (1:100) followed by goat anti-rabbit-AlexaFluor-488 secondary antibody (green). B. Merge of A and Live view of the cells.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Expression of Orai1 in rat pancreas.Immunohistochemical staining of rat pancreas paraffin embedded sections using Anti-Orai1 (extracellular) antibody, (1:50). Orai1 (brown) is expressed in acinar cells. Hematoxilin is used as the counterstain.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Indirect flow cytometry analysis of WEHI living cells: black line, Unstained cells. green line, Cells + Anti-Orai1 (extracellular) antibody, (10 ug/0.5 x 106 cells).
- Validation comment
- FC
