MAB328

antibody from Novus Biologicals

Targeting: CCL16 CKb12, HCC-4, LCC-1, LEC, LMC, Mtn-1, NCC-4, SCYA16, SCYL4

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB328

Provider

Novus Biologicals

Product name

Mouse Monoclonal CCL16/HCC-4/LEC Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from ascites. Detects human CCL16/HCC-4 in direct ELISAs and Western blots.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

500 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

CCL16/LEC powerfully triggers effector and antigen-presenting functions of macrophages and enhances T cell cytotoxicity.

Cappello P, Caorsi C, Bosticardo M, De Angelis S, Novelli F, Forni G, Giovarelli M
Journal of leukocyte biology 2004 Jan;75(1):135-42

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Chemotaxis Induced by CCL16/HCC-4 and Neutral-ization by Human CCL16/ HCC-4 Antibody. Recombinant Human CCL16/ HCC-4 (Catalog # 802-HC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL16/ HCC-4 (0.75 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL16/ HCC-4 Monoclonal Antibody (Catalog # MAB328). The ND₅₀ is typically 5-20 µg/mL.