500-P125G-1MG
antibody from Invitrogen Antibodies
Targeting: CCL16
CKb12, HCC-4, LCC-1, LEC, LMC, Mtn-1, NCC-4, SCYA16, SCYL4
Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
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Validation data
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- Product number
- 500-P125G-1MG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CCL16 Polyclonal Antibody, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: QPKVPEWVNT PSTCCLKYYE KVLPRRLVVG YRKALNCHLP AIIFVTKRNR EVCTNPNDDW VQEYIKDPNL PLLPTRNLST VKIITAKNGQ PQLLNSQ.
- Concentration
- 0.1-1.0 mg/mL
Submitted references Tumor rejection and immune memory elicited by locally released LEC chemokine are associated with an impressive recruitment of APCs, lymphocytes, and granulocytes.
LEC induces chemotaxis and adhesion by interacting with CCR1 and CCR8.
Giovarelli M, Cappello P, Forni G, Salcedo T, Moore PA, LeFleur DW, Nardelli B, Di Carlo E, Lollini PL, Ruben S, Ullrich S, Garotta G, Musiani P
Journal of immunology (Baltimore, Md. : 1950) 2000 Mar 15;164(6):3200-6
Journal of immunology (Baltimore, Md. : 1950) 2000 Mar 15;164(6):3200-6
LEC induces chemotaxis and adhesion by interacting with CCR1 and CCR8.
Howard OM, Dong HF, Shirakawa AK, Oppenheim JJ
Blood 2000 Aug 1;96(3):840-5
Blood 2000 Aug 1;96(3):840-5
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect Human LEC (CCL16) by Western Blot analysis CCL16 Polyclonal Antibody (Product # 500-P125G-1MG) can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human LEC (CCL16) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect Human LEC (CCL16) by Western Blot analysis CCL16 Polyclonal Antibody (Product # 500-P125G-1MG) can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human LEC (CCL16) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA: To detect Human LEC (CCL16) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of CCL16 Polyclonal Antibody (Product # 500-P125G-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech CCL16 Polyclonal Antibody, Biotin (Product # 500-P125GBT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human (CCL16) LEC.