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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PAB3629 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3629, RRID:AB_1579394
- Product name
- PTPRC polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PTPRC.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references A high-frequency polymorphism in exon 6 of the CD45 tyrosine phosphatase gene (PTPRC) resulting in altered isoform expression.
77 C/G mutation in the tyrosine phosphatase CD45 gene and autoimmune hepatitis: evidence for a genetic link.
Negative regulation of T cell activation by placental protein 14 is mediated by the tyrosine phosphatase receptor CD45.
Stanton T, Boxall S, Hirai K, Dawes R, Tonks S, Yasui T, Kanaoka Y, Yuldasheva N, Ishiko O, Bodmer W, Beverley PC, Tchilian EZ
Proceedings of the National Academy of Sciences of the United States of America 2003 May 13;100(10):5997-6002
Proceedings of the National Academy of Sciences of the United States of America 2003 May 13;100(10):5997-6002
77 C/G mutation in the tyrosine phosphatase CD45 gene and autoimmune hepatitis: evidence for a genetic link.
Vogel A, Strassburg CP, Manns MP
Genes and immunity 2003 Jan;4(1):79-81
Genes and immunity 2003 Jan;4(1):79-81
Negative regulation of T cell activation by placental protein 14 is mediated by the tyrosine phosphatase receptor CD45.
Rachmilewitz J, Borovsky Z, Riely GJ, Miller R, Tykocinski ML
The Journal of biological chemistry 2003 Apr 18;278(16):14059-65
The Journal of biological chemistry 2003 Apr 18;278(16):14059-65
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The PTPRC polyclonal antibody (Cat # PAB3629) is used in Western blot to detect PTPRC in Jurkat cell lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human tonsil reacted with PTPRC polyclonal antibody (Cat # PAB3629), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells using PTPRC polyclonal antibody (Cat # PAB3629) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- Flow Cytometry
