- NBP1-30141 - Novus Biologicals H3C14 antibody

Submitted references High methylation levels of histone H3 lysine 9 associated with activation of hypoxia-inducible factor 1α (HIF-1α) predict patients' worse prognosis in human hepatocellular carcinomas.

Qian Y, Li Y, Zheng C, Lu T, Sun R, Mao Y, Yu S, Fan H, Zhang Z
Cancer genetics 2020 Jul;245:17-26

Mechanical stress impairs pheromone signaling via Pkc1-mediated regulation of the MAPK scaffold Ste5.

van Drogen F, Mishra R, Rudolf F, Walczak MJ, Lee SS, Reiter W, Hegemann B, Pelet S, Dohnal I, Binolfi A, Yudina Z, Selenko P, Wider G, Ammerer G, Peter M
The Journal of cell biology 2019 Sep 2;218(9):3117-3133

Vac8 spatially confines autophagosome formation at the vacuole in S. cerevisiae.

Hollenstein DM, Gómez-Sánchez R, Ciftci A, Kriegenburg F, Mari M, Torggler R, Licheva M, Reggiori F, Kraft C
Journal of cell science 2019 Nov 14;132(22)

UVA irradiation strengthened an interaction between UBF1/2 proteins and H4K20 di-/tri-methylation.

Stixová L, Komůrková D, Svobodová Kovaříková A, Bártová E
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology 2019 Mar;27(1-2):41-55

Methylation Patterns of Lys9 and Lys27 on Histone H3 Correlate with Patient Outcome in Gastric Cancer.

Li Y, Guo D, Sun R, Chen P, Qian Q, Fan H
Digestive diseases and sciences 2019 Feb;64(2):439-446

H3K9me3, H3K36me3, and H4K20me3 Expression Correlates with Patient Outcome in Esophageal Squamous Cell Carcinoma as Epigenetic Markers.

Zhou M, Li Y, Lin S, Chen Y, Qian Y, Zhao Z, Fan H
Digestive diseases and sciences 2019 Aug;64(8):2147-2157

A medial prefrontal cortex-nucleus acumens corticotropin-releasing factor circuitry for neuropathic pain-increased susceptibility to opioid reward.

Kai Y, Li Y, Sun T, Yin W, Mao Y, Li J, Xie W, Chen S, Wang L, Li J, Zhang Z, Tao W
Translational psychiatry 2018 May 21;8(1):100

NF-κB inhibition rescues cardiac function by remodeling calcium genes in a Duchenne muscular dystrophy model.

Peterson JM, Wang DJ, Shettigar V, Roof SR, Canan BD, Bakkar N, Shintaku J, Gu JM, Little SC, Ratnam NM, Londhe P, Lu L, Gaw CE, Petrosino JM, Liyanarachchi S, Wang H, Janssen PML, Davis JP, Ziolo MT, Sharma SM, Guttridge DC
Nature communications 2018 Aug 24;9(1):3431

DZNep and UNC0642 enhance in vitro developmental competence of cloned pig embryos.

Zhao C, Shi J, Zhou R, He X, Yang H, Wu Z
Reproduction (Cambridge, England) 2018 Apr 1;157(4):359-369

Identifying protein kinase-specific effectors of the osmostress response in yeast.

Romanov N, Hollenstein DM, Janschitz M, Ammerer G, Anrather D, Reiter W
Science signaling 2017 Mar 7;10(469)

Vorinostat differentially alters 3D nuclear structure of cancer and non-cancerous esophageal cells.

Nandakumar V, Hansen N, Glenn HL, Han JH, Helland S, Hernandez K, Senechal P, Johnson RH, Bussey KJ, Meldrum DR
Scientific reports 2016 Aug 9;6:30593

Cell-free extract from porcine induced pluripotent stem cells can affect porcine somatic cell nuclear reprogramming.

No JG, Choi MK, Kwon DJ, Yoo JG, Yang BC, Park JK, Kim DH
The Journal of reproduction and development 2015;61(2):90-8

The TRPM7 chanzyme is cleaved to release a chromatin-modifying kinase.

Krapivinsky G, Krapivinsky L, Manasian Y, Clapham DE
Cell 2014 May 22;157(5):1061-72

Chorion formation in panoistic ovaries requires windei and trimethylation of histone 3 lysine 9.

Herraiz A, Belles X, Piulachs MD
Experimental cell research 2014 Jan 1;320(1):46-53

Mouse Rif1 is a key regulator of the replication-timing programme in mammalian cells.

Cornacchia D, Dileep V, Quivy JP, Foti R, Tili F, Santarella-Mellwig R, Antony C, Almouzni G, Gilbert DM, Buonomo SB
The EMBO journal 2012 Sep 12;31(18):3678-90

Pharmacologic disruption of Polycomb-repressive complex 2-mediated gene repression selectively induces apoptosis in cancer cells.

Tan J, Yang X, Zhuang L, Jiang X, Chen W, Lee PL, Karuturi RK, Tan PB, Liu ET, Yu Q
Genes & development 2007 May 1;21(9):1050-63

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - H3K9Me3 analysis of MCF7 and HCT116. Theorectical molecular weight is ~15 kDa. Image from verified customer review.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Histone h3 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Observed molecular weight is ~15 kDa.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Analysis of Histone H3 (NBP1-30141) using HeLa nuclear lysate [NB800-PC9]. Observed molecular weight ~15 kDa.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Analysis of Histone H3 K9-me3 in HeLa histone lysates. Observed molecular weight is ~15 kDa.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Representative Western blot showing M-track protein protein proximity signals obtained for Kic1. Hog1-protA-H3: background control, Nup2: positive control. Below: Proximity signals. n = 3 replicates per sample except when indicated differently. Ratios are log2-transformed. Black lines indicate average proximity signal. Proximity signals that differ significantly from background are marked in green (q 0.05. N: N-terminal HKMTmyc fusion. Image collected and cropped by CiteAb from the following publication (https://biosignaling.biomedcentral.com/articles/10.1186/s12964-019-0381-z), licensed under a CC-BY licence.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Histone 3 was detected in immersion fixed paraffin-embedded sections of human colon cancer using anti-human mouse monoclonal antibody (Catalog # NBP1-30141) at 1:1000 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Used to perform ChIP on HeLa cells.

NBP1-30141

Antibody data