M00254

antibody from Boster Biological Technology

Targeting: MKI67 MIB-1, PPP1R105

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

M00254

Provider

Boster Biological Technology

Product name

Anti-Ki67 MKI67 Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for KI67/MKI67 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IF, IHC, ICC, WB. Reactive species: Human KI67/MKI67 information: Molecular Weight: 358694 MW; Subcellular Localization: Nucleus . Nucleus, nucleolus . Chromosome . Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes.

Reactivity

Human

Host

Rabbit

Antibody clone number

EAH-13

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human tonsil, using Ki67 Antibody (M00254)MKI67 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MKI67 Antibody (M00254)overnight at 4?? Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?? The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image