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Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [31]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- V2003 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- PSA Antibody Prostate Specific Antigen
- Antibody type
- Monoclonal
- Antigen
- Prostate Specific Antigen from human sperm plasma was used as the antigen for this PSA antibody.
- Description
- Protein G purified antibody
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- A67-B/E3
- Vial size
- 20 µg, 100 µg (with sodium azide), 100 µg (without sodium azide), 7 ml (prediluted for IHC use, if applicable)
- Storage
- Store the PSA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Submitted references A concentration−dependent multicolor conversion strategy for ultrasensitive colorimetric immunoassay with the naked eye
An efficient method for native protein purification in the selected range from prostate cancer tissue digests.
The N-terminal domain of the androgen receptor drives its nuclear localization in castration-resistant prostate cancer cells.
Characterisation of a label-free biosensor based on long period grating.
Two-color SERS microscopy for protein co-localization in prostate tissue with primary antibody-protein A/G-gold nanocluster conjugates.
The receptor antagonist picotamide inhibits adrenergic and thromboxane-induced contraction of hyperplastic human prostate smooth muscle.
Phenotypic characterization of human prostatic stromal cells in primary cultures derived from human tissue samples.
The cAMP effector EPAC activates Elk1 transcription factor in prostate smooth muscle, and is a minor regulator of α1-adrenergic contraction.
ARF represses androgen receptor transactivation in prostate cancer.
Direct regulation of androgen receptor activity by potent CYP17 inhibitors in prostate cancer cells.
Characterization of the low pH/low nutrient-resistant LNCaP cell subline LNCaP-F10.
Effect of electroporation-mediated diphtheria toxin A expression on PSA positive human prostate xenograft tumors in SCID mice.
BTG2 is an LXXLL-dependent co-repressor for androgen receptor transcriptional activity.
GLI1, a crucial mediator of sonic hedgehog signaling in prostate cancer, functions as a negative modulator for androgen receptor.
CD90/THY1 is overexpressed in prostate cancer-associated fibroblasts and could serve as a cancer biomarker.
Gene expression down-regulation in CD90+ prostate tumor-associated stromal cells involves potential organ-specific genes.
Gene expression relationship between prostate cancer cells of Gleason 3, 4 and normal epithelial cells as revealed by cell type-specific transcriptomes.
Hormonal oscillations during the estrous cycle influence the morphophysiology of the gerbil (Meriones unguiculatus) female prostate (skene paraurethral glands).
Anion exchange fractionation of serum proteins versus albumin elimination.
Comparison of prostate cancer cell lines for androgen receptor-mediated reporter gene assays.
Modulation of androgen receptor transactivation by FoxH1. A newly identified androgen receptor corepressor.
p300 regulates androgen receptor-independent expression of prostate-specific antigen in prostate cancer cells treated chronically with interleukin-6.
Analysis of prostate cancer by proteomics using tissue specimens.
The effect of seminal vesicle-sparing radical prostatectomy on serum prostate-specific antigen level.
p300 mediates androgen-independent transactivation of the androgen receptor by interleukin 6.
Characterization of a novel androgen receptor mutation in a relapsed CWR22 prostate cancer xenograft and cell line.
Unusual alternative splicing within the human kallikrein genes KLK2 and KLK3 gives rise to novel prostate-specific proteins.
Androgen receptor expression and cellular proliferation during transition from androgen-dependent to recurrent growth after castration in the CWR22 prostate cancer xenograft.
Morphological and immunohistochemical characteristics of the heterogeneous prostate-like glands (paraurethral gland) seen in female Brown-Norway rats.
Induction of immune responses and safety profiles in rhesus macaques immunized with a DNA vaccine expressing human prostate specific antigen.
Characterization of PSA-RP2, a protein related to prostate-specific antigen and encoded by alternative hKLK3 transcripts.
Liu Y, Zhang Z, Yu J, Xie J, Li C
Analytica Chimica Acta 2017 April;963
Analytica Chimica Acta 2017 April;963
An efficient method for native protein purification in the selected range from prostate cancer tissue digests.
Ahmad R, Nicora CD, Shukla AK, Smith RD, Qian WJ, Liu AY
Chinese clinical oncology 2016 Dec;5(6):78
Chinese clinical oncology 2016 Dec;5(6):78
The N-terminal domain of the androgen receptor drives its nuclear localization in castration-resistant prostate cancer cells.
Dar JA, Masoodi KZ, Eisermann K, Isharwal S, Ai J, Pascal LE, Nelson JB, Wang Z
The Journal of steroid biochemistry and molecular biology 2014 Sep;143:473-80
The Journal of steroid biochemistry and molecular biology 2014 Sep;143:473-80
Characterisation of a label-free biosensor based on long period grating.
Chiavaioli F, Trono C, Giannetti A, Brenci M, Baldini F
Journal of biophotonics 2014 May;7(5):312-22
Journal of biophotonics 2014 May;7(5):312-22
Two-color SERS microscopy for protein co-localization in prostate tissue with primary antibody-protein A/G-gold nanocluster conjugates.
Salehi M, Schneider L, Ströbel P, Marx A, Packeisen J, Schlücker S
Nanoscale 2014 Feb 21;6(4):2361-7
Nanoscale 2014 Feb 21;6(4):2361-7
The receptor antagonist picotamide inhibits adrenergic and thromboxane-induced contraction of hyperplastic human prostate smooth muscle.
Hennenberg M, Miljak M, Herrmann D, Strittmatter F, Walther S, Rutz B, Hocaoglu Y, Kunit T, Schreiber A, Andersson KE, Stief CG, Gratzke C
American journal of physiology. Renal physiology 2013 Nov 15;305(10):F1383-90
American journal of physiology. Renal physiology 2013 Nov 15;305(10):F1383-90
Phenotypic characterization of human prostatic stromal cells in primary cultures derived from human tissue samples.
Gravina GL, Mancini A, Ranieri G, Di Pasquale B, Marampon F, Di Clemente L, Ricevuto E, Festuccia C
International journal of oncology 2013 Jun;42(6):2116-22
International journal of oncology 2013 Jun;42(6):2116-22
The cAMP effector EPAC activates Elk1 transcription factor in prostate smooth muscle, and is a minor regulator of α1-adrenergic contraction.
Hennenberg M, Strittmatter F, Schmetkamp H, Rutz B, Walther S, Stief CG, Gratzke C
Journal of biomedical science 2013 Jul 2;20:46
Journal of biomedical science 2013 Jul 2;20:46
ARF represses androgen receptor transactivation in prostate cancer.
Lu W, Xie Y, Ma Y, Matusik RJ, Chen Z
Molecular endocrinology (Baltimore, Md.) 2013 Apr;27(4):635-48
Molecular endocrinology (Baltimore, Md.) 2013 Apr;27(4):635-48
Direct regulation of androgen receptor activity by potent CYP17 inhibitors in prostate cancer cells.
Soifer HS, Souleimanian N, Wu S, Voskresenskiy AM, Collak FK, Cinar B, Stein CA
The Journal of biological chemistry 2012 Feb 3;287(6):3777-87
The Journal of biological chemistry 2012 Feb 3;287(6):3777-87
Characterization of the low pH/low nutrient-resistant LNCaP cell subline LNCaP-F10.
Iguchi K, Hayakawa Y, Ishii K, Matsumoto K, Usui S, Sugimura Y, Hirano K
Oncology reports 2012 Dec;28(6):2009-15
Oncology reports 2012 Dec;28(6):2009-15
Effect of electroporation-mediated diphtheria toxin A expression on PSA positive human prostate xenograft tumors in SCID mice.
Goepfert C, Gazdhar A, Frey FJ, Frey BM
The Prostate 2011 Jun 1;71(8):872-80
The Prostate 2011 Jun 1;71(8):872-80
BTG2 is an LXXLL-dependent co-repressor for androgen receptor transcriptional activity.
Hu XD, Meng QH, Xu JY, Jiao Y, Ge CM, Jacob A, Wang P, Rosen EM, Fan S
Biochemical and biophysical research communications 2011 Jan 28;404(4):903-9
Biochemical and biophysical research communications 2011 Jan 28;404(4):903-9
GLI1, a crucial mediator of sonic hedgehog signaling in prostate cancer, functions as a negative modulator for androgen receptor.
Chen G, Goto Y, Sakamoto R, Tanaka K, Matsubara E, Nakamura M, Zheng H, Lu J, Takayanagi R, Nomura M
Biochemical and biophysical research communications 2011 Jan 21;404(3):809-15
Biochemical and biophysical research communications 2011 Jan 21;404(3):809-15
CD90/THY1 is overexpressed in prostate cancer-associated fibroblasts and could serve as a cancer biomarker.
True LD, Zhang H, Ye M, Huang CY, Nelson PS, von Haller PD, Tjoelker LW, Kim JS, Qian WJ, Smith RD, Ellis WJ, Liebeskind ES, Liu AY
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2010 Oct;23(10):1346-56
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2010 Oct;23(10):1346-56
Gene expression down-regulation in CD90+ prostate tumor-associated stromal cells involves potential organ-specific genes.
Pascal LE, Goo YA, Vêncio RZ, Page LS, Chambers AA, Liebeskind ES, Takayama TK, True LD, Liu AY
BMC cancer 2009 Sep 8;9:317
BMC cancer 2009 Sep 8;9:317
Gene expression relationship between prostate cancer cells of Gleason 3, 4 and normal epithelial cells as revealed by cell type-specific transcriptomes.
Pascal LE, Vêncio RZ, Page LS, Liebeskind ES, Shadle CP, Troisch P, Marzolf B, True LD, Hood LE, Liu AY
BMC cancer 2009 Dec 18;9:452
BMC cancer 2009 Dec 18;9:452
Hormonal oscillations during the estrous cycle influence the morphophysiology of the gerbil (Meriones unguiculatus) female prostate (skene paraurethral glands).
Fochi RA, Perez AP, Bianchi CV, Rochel SS, Góes RM, Vilamaior PS, Taboga SR, Santos FC
Biology of reproduction 2008 Dec;79(6):1084-91
Biology of reproduction 2008 Dec;79(6):1084-91
Anion exchange fractionation of serum proteins versus albumin elimination.
Sahab ZJ, Iczkowski KA, Sang QX
Analytical biochemistry 2007 Sep 1;368(1):24-32
Analytical biochemistry 2007 Sep 1;368(1):24-32
Comparison of prostate cancer cell lines for androgen receptor-mediated reporter gene assays.
Kim HJ, Park YI, Dong MS
Toxicology in vitro : an international journal published in association with BIBRA 2006 Oct;20(7):1159-67
Toxicology in vitro : an international journal published in association with BIBRA 2006 Oct;20(7):1159-67
Modulation of androgen receptor transactivation by FoxH1. A newly identified androgen receptor corepressor.
Chen G, Nomura M, Morinaga H, Matsubara E, Okabe T, Goto K, Yanase T, Zheng H, Lu J, Nawata H
The Journal of biological chemistry 2005 Oct 28;280(43):36355-63
The Journal of biological chemistry 2005 Oct 28;280(43):36355-63
p300 regulates androgen receptor-independent expression of prostate-specific antigen in prostate cancer cells treated chronically with interleukin-6.
Debes JD, Comuzzi B, Schmidt LJ, Dehm SM, Culig Z, Tindall DJ
Cancer research 2005 Jul 1;65(13):5965-73
Cancer research 2005 Jul 1;65(13):5965-73
Analysis of prostate cancer by proteomics using tissue specimens.
Liu AY, Zhang H, Sorensen CM, Diamond DL
The Journal of urology 2005 Jan;173(1):73-8
The Journal of urology 2005 Jan;173(1):73-8
The effect of seminal vesicle-sparing radical prostatectomy on serum prostate-specific antigen level.
John H, Hauri D, Maake C
BJU international 2003 Dec;92(9):920-3
BJU international 2003 Dec;92(9):920-3
p300 mediates androgen-independent transactivation of the androgen receptor by interleukin 6.
Debes JD, Schmidt LJ, Huang H, Tindall DJ
Cancer research 2002 Oct 15;62(20):5632-6
Cancer research 2002 Oct 15;62(20):5632-6
Characterization of a novel androgen receptor mutation in a relapsed CWR22 prostate cancer xenograft and cell line.
Tepper CG, Boucher DL, Ryan PE, Ma AH, Xia L, Lee LF, Pretlow TG, Kung HJ
Cancer research 2002 Nov 15;62(22):6606-14
Cancer research 2002 Nov 15;62(22):6606-14
Unusual alternative splicing within the human kallikrein genes KLK2 and KLK3 gives rise to novel prostate-specific proteins.
David A, Mabjeesh N, Azar I, Biton S, Engel S, Bernstein J, Romano J, Avidor Y, Waks T, Eshhar Z, Langer SZ, Lifschitz-Mercer B, Matzkin H, Rotman G, Toporik A, Savitsky K, Mintz L
The Journal of biological chemistry 2002 May 17;277(20):18084-90
The Journal of biological chemistry 2002 May 17;277(20):18084-90
Androgen receptor expression and cellular proliferation during transition from androgen-dependent to recurrent growth after castration in the CWR22 prostate cancer xenograft.
Kim D, Gregory CW, French FS, Smith GJ, Mohler JL
The American journal of pathology 2002 Jan;160(1):219-26
The American journal of pathology 2002 Jan;160(1):219-26
Morphological and immunohistochemical characteristics of the heterogeneous prostate-like glands (paraurethral gland) seen in female Brown-Norway rats.
Satoh H, Mori K, Furuhama K
Toxicologic pathology 2001 Mar-Apr;29(2):237-41
Toxicologic pathology 2001 Mar-Apr;29(2):237-41
Induction of immune responses and safety profiles in rhesus macaques immunized with a DNA vaccine expressing human prostate specific antigen.
Kim JJ, Yang JS, Nottingham LK, Tang W, Dang K, Manson KH, Wyand MS, Wilson DM, Weiner DB
Oncogene 2001 Jul 27;20(33):4497-506
Oncogene 2001 Jul 27;20(33):4497-506
Characterization of PSA-RP2, a protein related to prostate-specific antigen and encoded by alternative hKLK3 transcripts.
Heuzé-Vourc'h N, Leblond V, Olayat S, Gauthier F, Courty Y
European journal of biochemistry 2001 Aug;268(16):4408-13
European journal of biochemistry 2001 Aug;268(16):4408-13
No comments: Submit comment
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of human prostate carcinoma stained with PSA antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE Analysis of Purified, BSA-Free PSA Antibody (A67-B/E3). Confirmation of Integrity and Purity of the Antibody.
