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- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
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- Product number
- PAB9983 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB9983, RRID:AB_1672204
- Product name
- CTCFL polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of CTCFL.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references The novel BORIS + CTCF gene family is uniquely involved in the epigenetics of normal biology and cancer.
BORIS, a novel male germ-line-specific protein associated with epigenetic reprogramming events, shares the same 11-zinc-finger domain with CTCF, the insulator protein involved in reading imprinting marks in the soma.
Klenova EM, Morse HC 3rd, Ohlsson R, Lobanenkov VV
Seminars in cancer biology 2002 Oct;12(5):399-414
Seminars in cancer biology 2002 Oct;12(5):399-414
BORIS, a novel male germ-line-specific protein associated with epigenetic reprogramming events, shares the same 11-zinc-finger domain with CTCF, the insulator protein involved in reading imprinting marks in the soma.
Loukinov DI, Pugacheva E, Vatolin S, Pack SD, Moon H, Chernukhin I, Mannan P, Larsson E, Kanduri C, Vostrov AA, Cui H, Niemitz EL, Rasko JE, Docquier FM, Kistler M, Breen JJ, Zhuang Z, Quitschke WW, Renkawitz R, Klenova EM, Feinberg AP, Ohlsson R, Morse HC 3rd, Lobanenkov VV
Proceedings of the National Academy of Sciences of the United States of America 2002 May 14;99(10):6806-11
Proceedings of the National Academy of Sciences of the United States of America 2002 May 14;99(10):6806-11
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot using CTCFL polyclonal antibody (Cat # PAB9983) shows detection of a predominant band corresponding to BORIS in human tissue lysates (arrowhead).Lane 1 contains lysate from human prostate tissue.Lane 2 contains lysate from human spleen tissue.Apredominant band at ~75 KDa is observed.Molecular weight estimation was made by comparison to prestained MW markers as indicated.
