11074-2-AP

antibody from Invitrogen Antibodies

Targeting: CTCFL BORIS, CT27, dJ579F20.2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

11074-2-AP

Provider

Invitrogen Antibodies

Product name

BORIS Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Polyclonal anti-CFCFL antibody (11074-2-AP) is produced by immunizing animals with part of C-terminal chain of CTCFL and detects a 75 kDa and a 55 kDa band.

Concentration

0.29 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PC-3 cells were subjected to SDS PAGE followed by western blot with 11074-2-AP (BORIS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Rat testis tissue were subjected to SDS PAGE followed by western blot with 11074-2-AP (BORIS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse testis tissue were subjected to SDS PAGE followed by western blot with 11074-2-AP (BORIS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 11074-2-AP (BORIS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HeLa cells using 11074-2-AP (BORIS antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of SH-SY5Y7 cells, using CTCFL antibody 11074-2-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IGG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human ovary tumor using 11074-2-AP (BORIS antibody) at dilution of 1:50 (under 40x lens).