Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA1-16817 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NPC1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- By Western blot, PA1-16817 detects heterogeneously glycosylated NPC1 protein with prominent bands at ~170 and ~220 kDa.
- Concentration
- 1 mg/mL
Submitted references Tonic prime-boost of STING signalling mediates Niemann-Pick disease type C.
Multiple cationic amphiphiles induce a Niemann-Pick C phenotype and inhibit Ebola virus entry and infection.
Chu TT, Tu X, Yang K, Wu J, Repa JJ, Yan N
Nature 2021 Aug;596(7873):570-575
Nature 2021 Aug;596(7873):570-575
Multiple cationic amphiphiles induce a Niemann-Pick C phenotype and inhibit Ebola virus entry and infection.
Shoemaker CJ, Schornberg KL, Delos SE, Scully C, Pajouhesh H, Olinger GG, Johansen LM, White JM
PloS one 2013;8(2):e56265
PloS one 2013;8(2):e56265
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot detection of human NPC1 in 20 µg of human fibroblast cell lysate using Product # PA1-16817.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockout validation by Western blot analysis of NPC1 in lysates of HeLa human cervical epithelial carcinoma parental cell line and NPC1 knockout (KO) HeLa cell line. Samples were incubated in NPC1 polyclonal antibody (Product # PA1-16817) using a dilution of 1:1000 followed by a HRP-conjugated Anti-Rabbit IgG secondary antibody. Specific band was detected for NPC1 at approximately 240-260 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of NPC intracellular cholesterol transporter 1 was achieved by transfecting HeLa with NPC intracellular cholesterol transporter 1 specific siRNAs (Silencer® select Product # s9668, s9669). Western Blot analysis (Fig. a) was performed using Whole cell extracts from the NPC intracellular cholesterol transporter 1 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The Blot was probed with NPC1 Polyclonal Antibody (Product # PA1-16817, 1:1000 ) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000). Densitometric analysis of this western Blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to NPC intracellular cholesterol transporter 1.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot was performed using Anti-NPC1 Polyclonal Antibody (Product # PA1-16817) and a 180 kDa band corresponding to NPC intracellular cholesterol transporter 1 was observed across cell lines tested. Whole cell extracts (30 µg lysate) of U-87 MG (Lane 1), Hep G2 (Lane 2) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright FL 1000 (Product # A32752).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of NPC1 in HeLa cells. Samples were incubated in NPC1 polyclonal antibody (Product # PA1-16817) followed by DyLight 488 (green). Nuclei were counterstained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NPC1 in human brain, cortex, neurons and astrocytes. Samples were incubated in NPC1 polyclonal antibody (Product # PA1-16817).
