H00011236-M01

antibody from Abnova Corporation

Targeting: RNF139 HRCA1, RCA1, TRC8

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00011236-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00011236-M01, RRID:AB_489848

Product name

RNF139 monoclonal antibody (M01), clone 3D10

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant RNF139.

Antigen sequence

HYFHALCLRKWLYIQDTCPMCHQKVYIEDDIKDNS
NVSNNNGFIPPNETPEEAVREAAAESDRELNEDDS
TDCDDDVQRERNGVIQHTGAAAEEFNDDTD


Isotype

IgG

Antibody clone number

3D10

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

A meckelin-filamin A interaction mediates ciliogenesis.

Adams M, Simms RJ, Abdelhamed Z, Dawe HR, Szymanska K, Logan CV, Wheway G, Pitt E, Gull K, Knowles MA, Blair E, Cross SH, Sayer JA, Johnson CA
Human molecular genetics 2012 Mar 15;21(6):1272-86

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

RNF139 monoclonal antibody (M01), clone 3D10 Western Blot analysis of RNF139 expression in HepG2 ( Cat # L019V1 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged RNF139 is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to RNF139 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol