Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB2291 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2291, RRID:AB_1577185
- Product name
- MAP3K9 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of MAP3K9.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Phosphoregulation of mixed-lineage kinase 1 activity by multiple phosphorylation in the activation loop.
Identification and characterization of SPRK, a novel src-homology 3 domain-containing proline-rich kinase with serine/threonine kinase activity.
Identification of a new family of human epithelial protein kinases containing two leucine/isoleucine-zipper domains.
Durkin JT, Holskin BP, Kopec KK, Reed MS, Spais CM, Steffy BM, Gessner G, Angeles TS, Pohl J, Ator MA, Meyer SL
Biochemistry 2004 Dec 28;43(51):16348-55
Biochemistry 2004 Dec 28;43(51):16348-55
Identification and characterization of SPRK, a novel src-homology 3 domain-containing proline-rich kinase with serine/threonine kinase activity.
Gallo KA, Mark MR, Scadden DT, Wang Z, Gu Q, Godowski PJ
The Journal of biological chemistry 1994 May 27;269(21):15092-100
The Journal of biological chemistry 1994 May 27;269(21):15092-100
Identification of a new family of human epithelial protein kinases containing two leucine/isoleucine-zipper domains.
Dorow DS, Devereux L, Dietzsch E, De Kretser T
European journal of biochemistry / FEBS 1993 Apr 15;213(2):701-10
European journal of biochemistry / FEBS 1993 Apr 15;213(2):701-10
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The MAP3K9 polyclonal antibody (Cat # PAB2291) is used in Western blot to detect MAP3K9 in A-375 cell lysate (lane 1) and rat testis tissue lysate (lane 2) .
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the MAP3K9 polyclonal antibody (Cat # PAB2291) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)