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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- sc-5284 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-5284, RRID:AB_626760
- Product name
- Anti-BRAF
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
Submitted references BRAF inactivation drives aneuploidy by deregulating CRAF.
RGS14 is a multifunctional scaffold that integrates G protein and Ras/Raf MAPkinase signalling pathways.
B-raf alternative splicing is dispensable for development but required for learning and memory associated with the hippocampus in the adult mouse.
BRAF(V600E) efficient transformation and induction of microsatellite instability versus KRAS(G12V) induction of senescence markers in human colon cancer cells.
CRAF autophosphorylation of serine 621 is required to prevent its proteasome-mediated degradation.
Cellular Ser/Thr-kinase assays using generic peptide substrates.
A-raf and B-raf are dispensable for normal endochondral bone development, and parathyroid hormone-related peptide suppresses extracellular signal-regulated kinase activation in hypertrophic chondrocytes.
BRAFE600-associated senescence-like cell cycle arrest of human naevi
Kamata T, Hussain J, Giblett S, Hayward R, Marais R, Pritchard C
Cancer research 2010 Nov 1;70(21):8475-86
Cancer research 2010 Nov 1;70(21):8475-86
RGS14 is a multifunctional scaffold that integrates G protein and Ras/Raf MAPkinase signalling pathways.
Shu FJ, Ramineni S, Hepler JR
Cellular signalling 2010 Mar;22(3):366-76
Cellular signalling 2010 Mar;22(3):366-76
B-raf alternative splicing is dispensable for development but required for learning and memory associated with the hippocampus in the adult mouse.
Valluet A, Hmitou I, Davis S, Druillennec S, Larcher M, Laroche S, Eychène A
PloS one 2010 Dec 22;5(12):e15272
PloS one 2010 Dec 22;5(12):e15272
BRAF(V600E) efficient transformation and induction of microsatellite instability versus KRAS(G12V) induction of senescence markers in human colon cancer cells.
Oikonomou E, Makrodouli E, Evagelidou M, Joyce T, Probert L, Pintzas A
Neoplasia (New York, N.Y.) 2009 Nov;11(11):1116-31
Neoplasia (New York, N.Y.) 2009 Nov;11(11):1116-31
CRAF autophosphorylation of serine 621 is required to prevent its proteasome-mediated degradation.
Noble C, Mercer K, Hussain J, Carragher L, Giblett S, Hayward R, Patterson C, Marais R, Pritchard CA
Molecular cell 2008 Sep 26;31(6):862-72
Molecular cell 2008 Sep 26;31(6):862-72
Cellular Ser/Thr-kinase assays using generic peptide substrates.
Adams DG, Wang Y, Mak PA, Chyba J, Shalizi O, Matzen J, Anderson P, Smith TR, Garcia M, Welch GL, Claret EJ, Fink M, Orth AP, Caldwell JS, Brinker A
Current chemical genomics 2008 May 23;1:54-64
Current chemical genomics 2008 May 23;1:54-64
A-raf and B-raf are dispensable for normal endochondral bone development, and parathyroid hormone-related peptide suppresses extracellular signal-regulated kinase activation in hypertrophic chondrocytes.
Provot S, Nachtrab G, Paruch J, Chen AP, Silva A, Kronenberg HM
Molecular and cellular biology 2008 Jan;28(1):344-57
Molecular and cellular biology 2008 Jan;28(1):344-57
BRAFE600-associated senescence-like cell cycle arrest of human naevi
Chrysiis Michaloglou, Liesbeth C. W. Vredeveld, Maria S. Soengas, Christophe Denoyelle, Thomas Kuilman, Chantal M. A. M. van der Horst, Donné M. Majoor, Jerry W. Shay, Wolter J. Mooi, Daniel S. Peeper
Nature 2005 Aug;436(7051):720-724
Nature 2005 Aug;436(7051):720-724
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Lane 1
- Marker [kDa]: 220, 112, 84, 47, 32, 26, 16.8
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 84
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
- Validation comment
- Two independent antibodies targeting one protein yielding similar staining patterns. Staining pattern consistent with experimental and/or bioinformatic data.
