LS-C784265

antibody from LSBio

Targeting: BRAF BRAF1

Western blot (Enhanced validation)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C784265

Provider

LSBio

Product name

BRAF / B-Raf Antibody (clone OTI3A7, Carrier-free) LS-C784265

Antibody type

Monoclonal

Description

Purified from ascites.

Reactivity

Human, Rat, Canine, Simian

Host

Mouse

Isotype

IgG

Antibody clone number

OTI3A7

Storage

Store at -20°C. Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRAF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRAF.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot of extracts (35 ug) from 9 different cell lines by using anti-anti-BRAF monoclonal antibody.

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded Ovary tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded pancreas tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded breast tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded breast tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded colon tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded Ovary tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded pancreas tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).