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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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- Product number
- 60292-1-IG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RBM4 Monoclonal Antibody (3H3F12)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Immunogen sequence: MVKLFIGNL PREATEQEIR SLFEQYGKVL ECDIIKNYGF VHIEDKTAAE DAIRNLHHYK LHGVNINVEA SKNKSKTSTK LHVGNISPTC TNKELRAKFE EYGPVIECDI VKDYAFVHME RAEDAVEAIR GLDNTEFQGE PPSLGRGLNT RLCAENGWIS KRRGLVKITA VGWLVMKK (1-177 aa encoded by BC021120)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3H3F12
- Vial size
- 150 µL
- Concentration
- 1.45 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB result of RBM4 antibody (60292-1-IG; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM4 transfected HeLa cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 60292-1-IG ( RBM4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60292-1-Ig (RBM4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 60292-1-IG (RBM4 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 60292-1-Ig (RBM4 Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human stomach tissue slide using 60292-1-Ig (RBM4 Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human stomach tissue slide using 60292-1-Ig (RBM4 Antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 60292-1-IG ( RBM4 Antibody) at dilution of 1:50 (under 40x lens).
