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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- MAB9087-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human LYPD8 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human LYPD8 in direct ELISAs. Stains human LYPD8 transfectants but not irrelevant transfectants in flow cytometry.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q6UX82- Isotype
- IgG
- Antibody clone number
- 961703
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- LYPD8 in HEK293 Human Cell Line. LYPD8 was detected in immersion fixed HEK293 human embryonic kidney cell line using Mouse Anti-Human LYPD8 Monoclonal Antibody (Catalog # MAB9087) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of LYPD8 in HEK293 Human Cell Line Transfected with Human LYPD8 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human LYPD8 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human LYPD8 Monoclonal Antibody (Catalog # MAB9087) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.
