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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PAB1750 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB1750, RRID:AB_1238124
- Product name
- SQSTM1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of SQSTM1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Inhibition of autophagic turnover in β-cells by fatty acids and glucose leads to apoptotic cell death.
Absence of autophagy results in reactive oxygen species-dependent amplification of RLR signaling.
Impaired protein aggregate handling and clearance underlie the pathogenesis of p97/VCP-associated disease.
Mir SU, George NM, Zahoor L, Harms R, Guinn Z, Sarvetnick NE
The Journal of biological chemistry 2015 Mar 6;290(10):6071-85
The Journal of biological chemistry 2015 Mar 6;290(10):6071-85
Absence of autophagy results in reactive oxygen species-dependent amplification of RLR signaling.
Tal MC, Sasai M, Lee HK, Yordy B, Shadel GS, Iwasaki A
Proceedings of the National Academy of Sciences of the United States of America 2009 Feb 24;106(8):2770-5
Proceedings of the National Academy of Sciences of the United States of America 2009 Feb 24;106(8):2770-5
Impaired protein aggregate handling and clearance underlie the pathogenesis of p97/VCP-associated disease.
Ju JS, Miller SE, Hanson PI, Weihl CC
The Journal of biological chemistry 2008 Oct 31;283(44):30289-99
The Journal of biological chemistry 2008 Oct 31;283(44):30289-99
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of SQSTM1 (arrow) using SQSTM1 polyclonal antibody (Cat # PAB1750). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SQSTM1 gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence staining of SQSTM1 polyclonal antibody (Cat # PAB1750) on Methanol-fixed and PFA fixed HeLa cells. Data courtesy of Dr. Eeva-Liisa Eskelinen, University of Helsinki,Finland.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with SQSTM1 polyclonal antibody (Cat # PAB1750) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. Data courtesy of Dr. Eeva-Liisa Eskelinen, University of Helsinki,Finland.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
