LS-C675476

antibody from LSBio

Targeting: CCT2 Cctb

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-C675476

Provider

LSBio

Product name

CCT2 / CCT Beta Antibody LS-C675476

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot All Lanes:CCT2 antibody at 5.63ug/ml Lane 1:Jurkat whole cell lysate Lane 2:NIH3T3 whole cell lysate Lane 3:MCF-7 whole cell lysate Lane 4:K562 whole cell lysate Secondary Goat polyclonal to rabbit at 1/10000 dilution Predicted band size: 58,53 kDa Observed band size: 57 kDa

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitating CCT2 in Jurkat whole cell lysate Lane 1: Rabbit monoclonal IgG(1ug)instead of product in Jurkat whole cell lysate.For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000) Lane 2: product(4ug)+ Jurkat whole cell lysate(500ug) Lane 3: Jurkat whole cell lysate (20ug)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating CCT2 in Jurkat whole cell lysate Lane 1: Rabbit monoclonal IgG(1ug)instead of product in Jurkat whole cell lysate.For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000) Lane 2: product(4ug)+ Jurkat whole cell lysate(500ug) Lane 3: Jurkat whole cell lysate (20ug)