DM3500P

antibody from Acris Antibodies GmbH

Targeting: PDPN aggrus, Gp38, GP40, PA2.26, T1A-2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

DM3500P

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#DM3500P, RRID:AB_1007668

Product name

anti Podoplanin

Antibody type

Monoclonal

Antigen

GP36 (Podoplanin) expressed by MDCK cells.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

18H5

Vial size

0.1 mg

References

Submitted references

Expression of podoplanin and other lymphatic markers in the human anterior eye segment.

Birke K, Lütjen-Drecoll E, Kerjaschki D, Birke MT
Investigative ophthalmology & visual science 2010 Jan;51(1):344-54

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Multipotent mesenchymal stem cells acquire a lymphendothelial phenotype and enhance lymphatic regeneration in vivo.

Conrad C, Niess H, Huss R, Huber S, von Luettichau I, Nelson PJ, Ott HC, Jauch KW, Bruns CJ
Circulation 2009 Jan 20;119(2):281-9

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Wolbachia lipoprotein stimulates innate and adaptive immunity through Toll-like receptors 2 and 6 to induce disease manifestations of filariasis.

Turner JD, Langley RS, Johnston KL, Gentil K, Ford L, Wu B, Graham M, Sharpley F, Slatko B, Pearlman E, Taylor MJ
The Journal of biological chemistry 2009 Aug 14;284(33):22364-78

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Lymphatic reprogramming of microvascular endothelial cells by CEA-related cell adhesion molecule-1 via interaction with VEGFR-3 and Prox1.

Kilic N, Oliveira-Ferrer L, Neshat-Vahid S, Irmak S, Obst-Pernberg K, Wurmbach JH, Loges S, Kilic E, Weil J, Lauke H, Tilki D, Singer BB, Ergün S
Blood 2007 Dec 15;110(13):4223-33

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Expression of indoleamine 2,3-dioxygenase in tumor endothelial cells correlates with long-term survival of patients with renal cell carcinoma.

Riesenberg R, Weiler C, Spring O, Eder M, Buchner A, Popp T, Castro M, Kammerer R, Takikawa O, Hatz RA, Stief CG, Hofstetter A, Zimmermann W
Clinical cancer research : an official journal of the American Association for Cancer Research 2007 Dec 1;13(23):6993-7002

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot analysis of Podoplanin expression in Human lymphatic endothelial cells (LEC) and HUVECs. Total lysate of both cell types were subjected to SDS-PAGE and subsequent Western analysis with the Podoplanin Antibody Cat.-No DM3500P. The antibody recognizes a protein of about 36 kDa in total lysate from LECs but not from HUVEC.

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunofluorescence staining (green) of Podoplanin in primary human dermal lymphatic endothelial cells (HDLEC) with anti-Human Podoplanin Antibody VCat.-No DM3500P (0.5 μg/ml) and counter staining of nuclei with Dapi. As secondary antibody Goat anti-Mouse ALEXA Fluor 488 was used 1/400.

Flow cytometry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

FACS analysis with primary human dermal microvascular (HDMVEC) and umbilical vein (HUVEC) endothelial cells. The lymphatic endothelial cell marker Podoplanin is not expressed in the blood endothelial cells.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

FACS analysis with Human primary lymphatic endothelial cells (HDLEC). As secondary antibody anti-Mouse IgG-PE was used.