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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [2]
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- Product number
- DM3500P - Provider product page
- Provider
- OriGene
- Product name
- PDPN mouse monoclonal antibody, clone 18H5, Aff - Purified
- Antibody type
- Monoclonal
- Description
- PDPN mouse monoclonal antibody, clone 18H5, Aff - Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- PDPN
- Isotype
- IgG
- Antibody clone number
- 18H5
- Vial size
- 100 µg
- Concentration
- Lyophilized
Submitted references Lymphatic reprogramming of microvascular endothelial cells by CEA-related cell adhesion molecule-1 via interaction with VEGFR-3 and Prox1.
Kilic N, Oliveira-Ferrer L, Neshat-Vahid S, Irmak S, Obst-Pernberg K, Wurmbach JH, Loges S, Kilic E, Weil J, Lauke H, Tilki D, Singer BB, Ergün S
Blood 2007 Dec 15;110(13):4223-33
Blood 2007 Dec 15;110(13):4223-33
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of Podoplanin expression in Human lymphatic endothelial cells (LEC) and HUVECs. Total lysate of both cell types were subjected to SDS-PAGE and subsequent Western analysis with the Podoplanin?Antibody ?. The antibody recognizes a protein of about 36 kDa in total lysate from LECs but not from HUVEC.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescence staining (green) of Podoplanin in primary human dermal lymphatic endothelial cells (HDLEC) with anti-Human Podoplanin Antibody V (0.5 ug/ml) and counter staining of nuclei with Dapi. As secondary antibody Goat anti-Mouse ALEXA Fluor 488 was used 1/400.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- FACS analysis with primary human dermal microvascular (HDMVEC) and umbilical vein (HUVEC) endothelial cells. The lymphatic endothelial cell marker Podoplanin is not expressed in the blood endothelial cells.
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- FACS analysis with Human primary lymphatic endothelial cells (HDLEC). As secondary antibody anti-Mouse IgG-PE was used.
- Validation comment
- FC
