LS-B3455

antibody from LSBio

Targeting: MMP9 CLG4B

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-B3455

Provider

LSBio

Proper citation

LifeSpan Cat#LS-B3455, RRID:AB_2144883

Product name

IHC-plus™ MMP9 / Gelatinase B Antibody (aa644-673) LS-B3455

Antibody type

Polyclonal

Description

Ammonium sulfate precipitation

Reactivity

Human, Mouse

Host

Rabbit

Storage

Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western blot of lysate from NCI-H1299 cell line, using MMP9 Antibody (L659). Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35ug.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

MMP9 Antibody western blot of 293 cell line lysates (35 ug/lane). The MMP9 antibody detected the MMP9 protein (arrow).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western blot of MMP9 (arrow) in mouse spleen lysates (35 ug/lane). MMP9 (arrow) was detected using the purified antibody.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Anti-MMP-9 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.