Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MAB10810 - Provider product page
- Provider
- Abnova Corporation
- Product name
- MMP9 monoclonal antibody, clone 5G3
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant MMP9.
- Isotype
- IgG
- Antibody clone number
- 5G3
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using MMP9 monoclonal antibody, clone 5G3 (Cat # MAB10810) against recombinant protein.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using MMP9 monoclonal antibody, clone 5G3 (Cat # MAB10810) against HEK293 (1), MMP7-hIgGFc transfected HEK293 (2) cell lysate and MMP9-hIgGFc transfected HEK293 (3) cell lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELISA measurement of MMP9 monoclonal antibody, clone 5G3 (Cat # MAB10810).
- Validation comment
- ELISA
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of NIH/3T3 cells using MMP9 monoclonal antibody, clone 5G3 (Cat # MAB10810) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. MMP9 monoclonal antibody, clone 5G3 (Cat # MAB10810) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human brain tissues using MMP9 monoclonal antibody, clone 5G3 (Cat # MAB10810) with DAB staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HeLa cells using MMP9 monoclonal antibody, clone 5G3 (Cat # MAB10810) (blue) and negative control (red).
- Validation comment
- Flow Cytometry