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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Flow cytometry [1]
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- Product number
- IC9111F - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal MMP-9 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human MMP-9 in Flow Cytometry.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Submitted references Amphiregulin Regulates Phagocytosis-Induced Cell Death in Monocytes via EGFR and Matrix Metalloproteinases.
Differential Expression of Matrix Metalloproteinases 2, 9 and Cytokines by Neutrophils and Monocytes in the Clinical Forms of Chagas Disease.
MMP9 integrates multiple immunoregulatory pathways that discriminate high suppressive activity of human mesenchymal stem cells.
JAK/STAT regulation of Aspergillus fumigatus corneal infections and IL-6/23-stimulated neutrophil, IL-17, elastase, and MMP9 activity.
Development and characterization of a physiologically relevant model of lymphocyte migration in chronic lymphocytic leukemia.
Primary human acute myelogenous leukemia cells release matrix metalloproteases and their inhibitors: release profile and pharmacological modulation.
Platen C, Dreschers S, Reiss LK, Wappler J, Orlikowsky TW
Mediators of inflammation 2018;2018:4310419
Mediators of inflammation 2018;2018:4310419
Differential Expression of Matrix Metalloproteinases 2, 9 and Cytokines by Neutrophils and Monocytes in the Clinical Forms of Chagas Disease.
Medeiros NI, Fares RC, Franco EP, Sousa GR, Mattos RT, Chaves AT, Nunes MD, Dutra WO, Correa-Oliveira R, Rocha MO, Gomes JA
PLoS neglected tropical diseases 2017 Jan;11(1):e0005284
PLoS neglected tropical diseases 2017 Jan;11(1):e0005284
MMP9 integrates multiple immunoregulatory pathways that discriminate high suppressive activity of human mesenchymal stem cells.
Lavini-Ramos C, Silva HM, Soares-Schanoski A, Monteiro SM, Ferreira LRP, Pacanaro AP, Gomes S, Batista J, Faé K, Kalil J, Coelho V
Scientific reports 2017 Apr 13;7(1):874
Scientific reports 2017 Apr 13;7(1):874
JAK/STAT regulation of Aspergillus fumigatus corneal infections and IL-6/23-stimulated neutrophil, IL-17, elastase, and MMP9 activity.
Taylor PR, Roy S, Meszaros EC, Sun Y, Howell SJ, Malemud CJ, Pearlman E
Journal of leukocyte biology 2016 Jul;100(1):213-22
Journal of leukocyte biology 2016 Jul;100(1):213-22
Development and characterization of a physiologically relevant model of lymphocyte migration in chronic lymphocytic leukemia.
Walsby E, Buggins A, Devereux S, Jones C, Pratt G, Brennan P, Fegan C, Pepper C
Blood 2014 Jun 5;123(23):3607-17
Blood 2014 Jun 5;123(23):3607-17
Primary human acute myelogenous leukemia cells release matrix metalloproteases and their inhibitors: release profile and pharmacological modulation.
Reikvam H, Hatfield KJ, Oyan AM, Kalland KH, Kittang AO, Bruserud O
European journal of haematology 2010 Mar;84(3):239-51
European journal of haematology 2010 Mar;84(3):239-51
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of MMP-9 in NS0 Mouse Cell Line Transfected with Human MMP-9 by Flow Cytometry. NS0 mouse myeloma cell line transfected with human MMP-9 was stained with Mouse Anti-Human MMP-9 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC9111F, filled histogram) or isotype control antibody (Catalog # IC0041F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
