Antibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- BAF909 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse MMP-9 Biotinylated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse MMP-9 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) MMP-9 is observed and less than 1% cross-reactivity with rhMMP-1, -3, -7, -8, -10, -12, and -13 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Biotin
- Antigen sequence
P41245
- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Attenuation of myocardial injury in mice with functional deletion of the circadian rhythm gene mPer2.
Virag JA, Dries JL, Easton PR, Friesland AM, DeAntonio JH, Chintalgattu V, Cozzi E, Lehmann BD, Ding JM, Lust RM
American journal of physiology. Heart and circulatory physiology 2010 Mar;298(3):H1088-95
American journal of physiology. Heart and circulatory physiology 2010 Mar;298(3):H1088-95
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- MMP-9 in Mouse Splenocytes. MMP-9 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse MMP-9 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF909) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.