PAB29310

antibody from Abnova Corporation

Targeting: MMP9 CLG4B

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29310

Provider

Abnova Corporation

Product name

MMP9 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant human MMP9.

Antigen sequence

PRQRQSTLVLFPGDLRTNLTDRQLAEEYLYRYGYT
RVAEMRGESKSLGPALLLLQKQLSLPETGELDSAT
LKAMRTPRCGVPDLGRFQTFEGDLKWHHHNITYWI
QNYSEDLPRAVIDDAFARAFALWSAVTPLTFTRVY
SRDADI


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with MMP9 polyclonal antibody (Cat# PAB29310) at 1:100 - 1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence staining of U-2 OS cells with MMP9 polyclonal antibody (Cat# PAB29310) under 1-4 ug/mL working concentration.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human bone marrow tissue with MMP9 polyclonal antibody (Cat# PAB29310) at 1:500 - 1:1000 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)