Antibody data
- Antibody Data
- Antigen structure
- References [14]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [3]
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Validation data
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- Product number
- NBP2-13173 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal MMP-9 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Active MMP9. It does not react with the MMP9 proenzyme or the active and proenzyme forms of MMP2.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Localized Antileptin Therapy Prevents Aortic Root Dilatation and Preserves Left Ventricular Systolic Function in a Murine Model of Marfan Syndrome.
Non-Collagenous Dentin Protein Binding Sites Control Mineral Formation during the Biomineralisation Process in Radicular Dentin.
Upregulation of matrix metalloproteinase 9 (MMP9)/tissue inhibitor of metalloproteinase 1 (TIMP1) and MMP2/TIMP2 ratios may be involved in lipopolysaccharide-induced acute lung injury.
A role of PSA-NCAM in the survival of retinal ganglion cells (RGCs) after kainic acid damage.
Egg White-Derived Antihypertensive Peptide IRW (Ile-Arg-Trp) Reduces Blood Pressure in Spontaneously Hypertensive Rats via the ACE2/Ang (1-7)/Mas Receptor Axis.
Engineering a 3D-Bioprinted Model of Human Heart Valve Disease Using Nanoindentation-Based Biomechanics.
Egg White-Derived Antihypertensive Peptide IRW (Ile-Arg-Trp) Inhibits Angiotensin II-Stimulated Migration of Vascular Smooth Muscle Cells via Angiotensin Type I Receptor.
O-GlcNAcylation regulates breast cancer metastasis via SIRT1 modulation of FOXM1 pathway.
IGF1R as a Key Target in High Risk, Metastatic Medulloblastoma.
Clarithromycin regresses endometriotic implants in rat endometriosis model.
Effect of the non-specific matrix metalloproteinase inhibitor Doxycycline on endometriotic implants in an experimental rat model.
Heterogeneity of serum gelatinases MMP-2 and MMP-9 isoforms and charge variants.
Human matrix metalloproteinase-9: activation by limited trypsin treatment and generation of monoclonal antibodies specific for the activated form.
Human matrix metalloproteinase-9: activation by limited trypsin treatment and generation of monoclonal antibodies specific for the activated form.
Fisch S, Bachner-Hinenzon N, Ertracht O, Guo L, Arad Y, Ben-Zvi D, Liao R, Schneiderman J
Journal of the American Heart Association 2020 May 18;9(10):e014761
Journal of the American Heart Association 2020 May 18;9(10):e014761
Non-Collagenous Dentin Protein Binding Sites Control Mineral Formation during the Biomineralisation Process in Radicular Dentin.
Retana-Lobo C, Guerreiro-Tanomaru JM, Tanomaru-Filho M, Mendes de Souza BD, Reyes-Carmona J
Materials (Basel, Switzerland) 2020 Feb 27;13(5)
Materials (Basel, Switzerland) 2020 Feb 27;13(5)
Upregulation of matrix metalloproteinase 9 (MMP9)/tissue inhibitor of metalloproteinase 1 (TIMP1) and MMP2/TIMP2 ratios may be involved in lipopolysaccharide-induced acute lung injury.
Chen G, Ge D, Zhu B, Shi H, Ma Q
The Journal of international medical research 2020 Apr;48(4):300060520919592
The Journal of international medical research 2020 Apr;48(4):300060520919592
A role of PSA-NCAM in the survival of retinal ganglion cells (RGCs) after kainic acid damage.
Lobanovskaya N, Zharkovsky A
Neurotoxicology 2019 May;72:101-106
Neurotoxicology 2019 May;72:101-106
Egg White-Derived Antihypertensive Peptide IRW (Ile-Arg-Trp) Reduces Blood Pressure in Spontaneously Hypertensive Rats via the ACE2/Ang (1-7)/Mas Receptor Axis.
Liao W, Fan H, Davidge ST, Wu J
Molecular nutrition & food research 2019 May;63(9):e1900063
Molecular nutrition & food research 2019 May;63(9):e1900063
Engineering a 3D-Bioprinted Model of Human Heart Valve Disease Using Nanoindentation-Based Biomechanics.
van der Valk DC, van der Ven CFT, Blaser MC, Grolman JM, Wu PJ, Fenton OS, Lee LH, Tibbitt MW, Andresen JL, Wen JR, Ha AH, Buffolo F, van Mil A, Bouten CVC, Body SC, Mooney DJ, Sluijter JPG, Aikawa M, Hjortnaes J, Langer R, Aikawa E
Nanomaterials (Basel, Switzerland) 2018 May 3;8(5)
Nanomaterials (Basel, Switzerland) 2018 May 3;8(5)
Egg White-Derived Antihypertensive Peptide IRW (Ile-Arg-Trp) Inhibits Angiotensin II-Stimulated Migration of Vascular Smooth Muscle Cells via Angiotensin Type I Receptor.
Liao W, Fan H, Wu J
Journal of agricultural and food chemistry 2018 May 23;66(20):5133-5138
Journal of agricultural and food chemistry 2018 May 23;66(20):5133-5138
O-GlcNAcylation regulates breast cancer metastasis via SIRT1 modulation of FOXM1 pathway.
Ferrer CM, Lu TY, Bacigalupa ZA, Katsetos CD, Sinclair DA, Reginato MJ
Oncogene 2017 Jan 26;36(4):559-569
Oncogene 2017 Jan 26;36(4):559-569
IGF1R as a Key Target in High Risk, Metastatic Medulloblastoma.
Svalina MN, Kikuchi K, Abraham J, Lal S, Davare MA, Settelmeyer TP, Young MC, Peckham JL, Cho YJ, Michalek JE, Hernandez BS, Berlow NE, Jackson M, Guillaume DJ, Selden NR, Bigner DD, Nazemi KJ, Green SC, Corless CL, Gultekin S, Mansoor A, Rubin BP, Woltjer R, Keller C
Scientific reports 2016 Jun 3;6:27012
Scientific reports 2016 Jun 3;6:27012
Clarithromycin regresses endometriotic implants in rat endometriosis model.
Cavkaytar S, Tapisiz OL, Kiykac Altinbas S, Tapisiz A, Erdem O, Goktolga U
Journal of obstetrics and gynaecology : the journal of the Institute of Obstetrics and Gynaecology 2015;35(8):844-7
Journal of obstetrics and gynaecology : the journal of the Institute of Obstetrics and Gynaecology 2015;35(8):844-7
Effect of the non-specific matrix metalloproteinase inhibitor Doxycycline on endometriotic implants in an experimental rat model.
Goktolga U, Cavkaytar S, Altinbas SK, Tapisiz OL, Tapisiz A, Erdem O
Experimental and therapeutic medicine 2015 May;9(5):1813-1818
Experimental and therapeutic medicine 2015 May;9(5):1813-1818
Heterogeneity of serum gelatinases MMP-2 and MMP-9 isoforms and charge variants.
Rossano R, Larocca M, Riviello L, Coniglio MG, Vandooren J, Liuzzi GM, Opdenakker G, Riccio P
Journal of cellular and molecular medicine 2014 Feb;18(2):242-52
Journal of cellular and molecular medicine 2014 Feb;18(2):242-52
Human matrix metalloproteinase-9: activation by limited trypsin treatment and generation of monoclonal antibodies specific for the activated form.
Duncan ME, Richardson JP, Murray GI, Melvin WT, Fothergill JE
European journal of biochemistry 1998 Nov 15;258(1):37-43
European journal of biochemistry 1998 Nov 15;258(1):37-43
Human matrix metalloproteinase-9: activation by limited trypsin treatment and generation of monoclonal antibodies specific for the activated form.
Duncan ME, Richardson JP, Murray GI, Melvin WT, Fothergill JE
European journal of biochemistry 1998 Nov 15;258(1):37-43
European journal of biochemistry 1998 Nov 15;258(1):37-43
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MMP-9 Antibody (4A3) [NBP2-13173] - Analysis of activated MMP9 expression in MMP9 proenzyme incubated with trypsin for various times.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MMP-9 Antibody (4A3) [NBP2-13173] - 1-DZ analysis and identification of gelatinases by Western blot. 1.5 ul of serum from healthy control (HC) or from a patient with inactive RR-MS were loaded on a 7.5% polyacrylamide gel copolymerized with 0.1% (w/v) gelatin (1-DZ). For Western blot analysis, 2 ul of fivefold concentrated (Vivaspin 500, MWCO 50,000; GE Healthcare) serum samples (the same as in 1-DZ) were separated on a 7.5% polyacrylamide gel without gelatin (1-DE), transferred on nitrocellulose membranes and incubated with the following primary antibodies: Anti-NGAL Antibody (5G5) at concentration of 2.0 ug/ml; Anti-MMP-9 (Ab-8) Mouse mAb (IA5) at concentration of 2.66 ug/ml; Anti-MMP-2 (Ab-4) Mouse mAb (75-7F7) at concentration of 1.0 ug/ml and Anti-MMP-9 Ab (4A3) at concentration of 2.0 ug/ml. Image collected and cropped by CiteAb from the following publication (null), licensed under a CC-BY licence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MMP-9 Antibody (4A3) [NBP2-13173] - Identification of gelatinases by Western blot analysis. A pool of sera from 10 patients with inactive RR-MS was used. For 2-DZ, pooled sera were concentrated 10-fold and 40 ug of total proteins were applied (left panels). For Western blot analysis pooled sera were concentrated 40-fold and 160 ug of total proteins were subjected to 2-DE (right panels). 2-DE gels were cut and the corresponding nitrocellulose membranes were treated with anti-NGAL Ab (A'), anti-MMP-2 Ab (B') and Anti-MMP-9 (4A3) Ab, recognizing the 65 kD and the 82 kD activated forms (C'); Gel C show the region with isoelectric point between pH 4-5.5 and molecular mass between 85 and 55 kD, analysed by Western blot in C', respectively. Image collected and cropped by CiteAb from the following publication (null), licensed under a CC-BY licence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MMP-9 Antibody (4A3) [NBP2-13173] - Analysis of a FFPE section of human esophageal cancer using MMP9 antibody (clone 4A3) at 1:200. The staining was developed with HRP-labelled secondary antibody and DAB reagent followed by hematoxylin counterstaining. This antibody clone generated an extracellular and cytoplasmic staining mainly in the cancer cells while the signal was very weak in the stromal cells of the tumor.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MMP-9 Antibody (4A3) [NBP2-13173] - Analysis of a FFPE tissue section of human esophageal cancer using MMP9 antibody (clone 4A3) at 1:200. The staining was developed with HRP-labelled secondary antibody and DAB reagent followed by hematoxylin counterstaining. This antibody generated a specific extracellular and cytoplasmic staining primarily in the cancer cells while the signal was pretty weak in the tumor stroma.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: MMP-9 Antibody (4A3) [NBP2-13173] - MMP-9 (green) was detected in murine brain (cortex) using MMP-9-DyLight 488 (4A3) with a concentration of 1:20 in PBS for 2 hours. MMP9-Signals are overlaped with the corresponding phase contrast image. Image from verified customer review. Image using the FITC format of this antibody.