Antibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [3]
- Other assay [8]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-13199 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MMP9 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 1.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references TRIM66 Promotes Malignant Progression of Non-Small-Cell Lung Cancer Cells via Targeting MMP9.
Endothelial Thioredoxin-Interacting Protein Depletion Reduces Hemorrhagic Transformation in Hyperglycemic Mice after Embolic Stroke and Thrombolytic Therapy.
Inhibition of Matrix Metalloproteinases and Cancer Cell Detachment by Ru(II) Polypyridyl Complexes Containing 4,7-Diphenyl-1,10-phenanthroline Ligands-New Candidates for Antimetastatic Agents.
Cardiac Protective Effect of Kirenol against Doxorubicin-Induced Cardiac Hypertrophy in H9c2 Cells through Nrf2 Signaling via PI3K/AKT Pathways.
Transmembrane protein ADAM29 facilitates cell proliferation, invasion and migration in clear cell renal cell carcinoma.
CCL4 Inhibition in Atherosclerosis: Effects on Plaque Stability, Endothelial Cell Adhesiveness, and Macrophages Activation.
EphrinA1-Fc Attenuates Ventricular Remodeling and Dysfunction in Chronically Nonreperfused WT but not EphA2-R-M mice.
Expression of matrix metalloproteinase-9 and tumour necrosis factor-alpha in the synovial cells of patients with meniscus tears.
Characterization of articular cartilage homeostasis and the mechanism of superior cartilage regeneration of MRL/MpJ mice.
Forkhead box C2 promotes the invasion ability of human trophoblast cells through Hedgehog (Hh) signaling pathway.
Intranasal Curcumin Inhibits Pulmonary Fibrosis by Modulating Matrix Metalloproteinase-9 (MMP-9) in Ovalbumin-Induced Chronic Asthma.
Xu Y, Yang Q, Fang Z, Tan X, Zhang M, Chen W
Computational and mathematical methods in medicine 2022;2022:6058720
Computational and mathematical methods in medicine 2022;2022:6058720
Endothelial Thioredoxin-Interacting Protein Depletion Reduces Hemorrhagic Transformation in Hyperglycemic Mice after Embolic Stroke and Thrombolytic Therapy.
Salman M, Ismael S, Li L, Ahmed HA, Puchowicz MA, Ishrat T
Pharmaceuticals (Basel, Switzerland) 2021 Sep 27;14(10)
Pharmaceuticals (Basel, Switzerland) 2021 Sep 27;14(10)
Inhibition of Matrix Metalloproteinases and Cancer Cell Detachment by Ru(II) Polypyridyl Complexes Containing 4,7-Diphenyl-1,10-phenanthroline Ligands-New Candidates for Antimetastatic Agents.
Gajda-Morszewski P, Gurgul I, Janczy-Cempa E, Mazuryk O, Łomzik M, Brindell M
Pharmaceuticals (Basel, Switzerland) 2021 Oct 1;14(10)
Pharmaceuticals (Basel, Switzerland) 2021 Oct 1;14(10)
Cardiac Protective Effect of Kirenol against Doxorubicin-Induced Cardiac Hypertrophy in H9c2 Cells through Nrf2 Signaling via PI3K/AKT Pathways.
Alzahrani AM, Rajendran P, Veeraraghavan VP, Hanieh H
International journal of molecular sciences 2021 Mar 23;22(6)
International journal of molecular sciences 2021 Mar 23;22(6)
Transmembrane protein ADAM29 facilitates cell proliferation, invasion and migration in clear cell renal cell carcinoma.
Li SL, Jiang TQ, Cao QW, Liu SM
Journal of chemotherapy (Florence, Italy) 2021 Feb;33(1):40-50
Journal of chemotherapy (Florence, Italy) 2021 Feb;33(1):40-50
CCL4 Inhibition in Atherosclerosis: Effects on Plaque Stability, Endothelial Cell Adhesiveness, and Macrophages Activation.
Chang TT, Yang HY, Chen C, Chen JW
International journal of molecular sciences 2020 Sep 8;21(18)
International journal of molecular sciences 2020 Sep 8;21(18)
EphrinA1-Fc Attenuates Ventricular Remodeling and Dysfunction in Chronically Nonreperfused WT but not EphA2-R-M mice.
Whitehurst KS, Chan VA, Estes HK, Valsaraj S, Kent S, Sharma UM, Chase RC, Bhuiyan M, Virag JAI
International journal of molecular sciences 2020 Aug 13;21(16)
International journal of molecular sciences 2020 Aug 13;21(16)
Expression of matrix metalloproteinase-9 and tumour necrosis factor-alpha in the synovial cells of patients with meniscus tears.
Atiç R, Deveci E
Folia morphologica 2019;78(3):535-544
Folia morphologica 2019;78(3):535-544
Characterization of articular cartilage homeostasis and the mechanism of superior cartilage regeneration of MRL/MpJ mice.
Deng Z, Gao X, Sun X, Amra S, Lu A, Cui Y, Eltzschig HK, Lei G, Huard J
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Aug;33(8):8809-8821
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Aug;33(8):8809-8821
Forkhead box C2 promotes the invasion ability of human trophoblast cells through Hedgehog (Hh) signaling pathway.
Zhang Y, Zhang Y
Cell biology international 2018 Jul;42(7):859-866
Cell biology international 2018 Jul;42(7):859-866
Intranasal Curcumin Inhibits Pulmonary Fibrosis by Modulating Matrix Metalloproteinase-9 (MMP-9) in Ovalbumin-Induced Chronic Asthma.
Chauhan PS, Dash D, Singh R
Inflammation 2017 Feb;40(1):248-258
Inflammation 2017 Feb;40(1):248-258
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a MMP9 polyclonal antibody (Product # PA5-13199) in 293 cell lysates (35 µg per lane).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a MMP9 polyclonal antibody (Product # PA5-13199) in mouse spleen lysates (35 µg per lane).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed, paraffin-embedded human spleen tissue using a MMP9 polyclonal antibody (Product # PA5-13199), followed by HRP-conjugated secondary antibody and AEC staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed, paraffin-embedded human cancer tissue using a MMP9 polyclonal antibody (Product # PA5-13199), followed by HRP-conjugated secondary antibody and DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast carcinoma tissue using a MMP9 polyclonal antibody (Product # PA5-13199), followed by HRP-conjugated secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Effects of KRL on DOX-induced hypertrophy. H9c2 cells were treated for 24 h with 15 uM of KRL, 2 h before DOX (0.25 umol) treatment. ( A ) Representative Western blots showing changes in the protein levels of ANP and BNP. ( B ) KRL is illustrated to downregulate MMP9 and MMP2. Western blot analysis was performed to determine the total protein MMP9 and MMp2 levels in the total extract by including beta-actin as an internal loading control. ( C ) H9c2 cells were treated for 24 h with 10 and 15 uM of KRL, 2 h before the DOX (0.25 umol) treatment. The cells then underwent actin filament staining to observe the changes in the surface area of the cardiomyocytes. Scale bar indicated 100u m at 20x magnification. Data are represented as the mean +- SD of triplicate values ( n = 3) and * p < 0.05 represents significant variations compared with the control. # p < 0.05 represents significant variations as compared to DOX alone and KRL with DOX treatment groups.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 7 Expression Changes in MMP-2, MMP-9, TIMP1, DDR2 and (p)SMAD2/3/total SMAD2/3 in B6 and EphA2-R-M Mouse Hearts. MMP-9, MMP-2 and TIMP-1 expression in ephrinA1-Fc-treated +/-TGF-beta primary cardiac fibroblasts are modulated in a dose-dependent manner ( n = 3/group; significance: * vs. control, + vs. 0.1 uM EA1, ++ vs. TGF-beta). Western blots of DDR2 and (p)SMAD2/3/totalSMAD2/3 show dose-dependent changes in response to ephrinA1-Fc-treatment +/-TGF-beta (NS).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 The impact of STZ-induced sub-acute HG in EC-TXNIP KO on MMP-9, MMP-3 and VEGFA expression after eMCAO and tPA-reperfusion. ( A ) Representative Western blot images of MMP-9, MMP-3 and VEGFA. ( B ) Bar graphs. EC-TXNIP deletion significantly decreased the expression of MMP-9 compared with HG group with tPA treatment. A non-significant downregulation was observed in MMP-3 and VEGFA proteins. The optical density of protein bands was analyzed and normalized to beta-Actin (unpaired t -test). WT: wild-type; NG: normoglycemia; HG: hyperglycemia; tPA: tissue-type plasminogen activator; mean +- SD; ns = non-significant; * p < 0.05; n = 6.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 ( A ) The expression levels of the active form of MMP-9 and ( B ) Representative image of Western blot detection of MMP-9 activity measured in A549 cells after 24 h incubation with 1 or 4 uM of Ru complexes or GM6001, and 100 uM of NAMI-A. beta-actin was used for normalization. * p < 0.05.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 ( A ) MMP-9 localization profile in different cell compartments after incubating A549 cells for 24 h with 4 uM of [Ru(dip) 2 (bpy-SC)] 2+ or [Ru(dip) 2 (dpq)] 2+ . * p < 0.05. ( B ) Representative images of Western blot detection of MMP-9 activity in different cell compartments after incubating A549 cells for 24 h with 4 uM Ru complexes. The following notification for extracts was used: CEB--cytoplasmic, MEB--membrane, NEB1--soluble nuclear, NEB2--chromatin bound nuclear, PEB--cytoskeletal.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5. Effects of knockdown or overexpression of ADAM29 on proliferation- and motion-related proteins. Western blot assay was adopted to determine the effect of ADAM29 knockdown (a) or overexpression (b) on the level of Cyclin D1, PCNA, MMP9 and Snail. ** p < 0.01 vs. si-con group or vector group.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- The bindings between TRIM66 and MMP2 or MMP9. (a) Protein levels of MMP2 and MMP9 in each transfection group. (b) Cells were lysed and immunoprecipitated with antibodies, and immunocomplexes were analyzed by Western blot. (c) MMP9 level in proteins extracted from NSCLC and pulmonary alveolar epithelial cells.