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- Antibody Data
- Antigen structure
- References [11]
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- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- AF911 - Provider product page
- Provider
- R&D Systems
- Product name
- Human MMP-9 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human and mouse MMP-9 in Western blots, dot blots, and human MMP-9 by immunocytochemistry. In Western blots, less than 1% cross-reactivity with recombinant human MMP-1, -2, -3, -7, -8, -10, -12, and -13 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references EDTA/gelatin zymography method to identify C1s versus activated MMP-9 in plasma and immune complexes of patients with systemic lupus erythematosus.
RAGE mediates S100A7-induced breast cancer growth and metastasis by modulating the tumor microenvironment.
Plasma levels of the MMP-9:TIMP-1 complex as prognostic biomarker in breast cancer: a retrospective study.
Humanin, a cytoprotective peptide, is expressed in carotid atherosclerotic [corrected] plaques in humans.
GDF-15 is an inhibitor of leukocyte integrin activation required for survival after myocardial infarction in mice.
TRE17/USP6 oncogene translocated in aneurysmal bone cyst induces matrix metalloproteinase production via activation of NF-kappaB.
Fibroblast-conditioned media promote human sarcoma cell invasion.
Development and validation of sandwich ELISA microarrays with minimal assay interference.
Molecular profiling of cervical cancer progression.
Differential proteolytic enzyme activity in eosinophilic and neutrophilic asthma.
Differential expression and activity of matrix metalloproteinases during flow-modulated vein graft remodeling.
Ugarte-Berzal E, Martens E, Boon L, Vandooren J, Blockmans D, Proost P, Opdenakker G
Journal of cellular and molecular medicine 2019 Jan;23(1):576-585
Journal of cellular and molecular medicine 2019 Jan;23(1):576-585
RAGE mediates S100A7-induced breast cancer growth and metastasis by modulating the tumor microenvironment.
Nasser MW, Wani NA, Ahirwar DK, Powell CA, Ravi J, Elbaz M, Zhao H, Padilla L, Zhang X, Shilo K, Ostrowski M, Shapiro C, Carson WE 3rd, Ganju RK
Cancer research 2015 Mar 15;75(6):974-85
Cancer research 2015 Mar 15;75(6):974-85
Plasma levels of the MMP-9:TIMP-1 complex as prognostic biomarker in breast cancer: a retrospective study.
Thorsen SB, Christensen SL, Würtz SO, Lundberg M, Nielsen BS, Vinther L, Knowles M, Gee N, Fredriksson S, Møller S, Brünner N, Schrohl AS, Stenvang J
BMC cancer 2013 Dec 13;13:598
BMC cancer 2013 Dec 13;13:598
Humanin, a cytoprotective peptide, is expressed in carotid atherosclerotic [corrected] plaques in humans.
Zacharias DG, Kim SG, Massat AE, Bachar AR, Oh YK, Herrmann J, Rodriguez-Porcel M, Cohen P, Lerman LO, Lerman A
PloS one 2012;7(2):e31065
PloS one 2012;7(2):e31065
GDF-15 is an inhibitor of leukocyte integrin activation required for survival after myocardial infarction in mice.
Kempf T, Zarbock A, Widera C, Butz S, Stadtmann A, Rossaint J, Bolomini-Vittori M, Korf-Klingebiel M, Napp LC, Hansen B, Kanwischer A, Bavendiek U, Beutel G, Hapke M, Sauer MG, Laudanna C, Hogg N, Vestweber D, Wollert KC
Nature medicine 2011 May;17(5):581-8
Nature medicine 2011 May;17(5):581-8
TRE17/USP6 oncogene translocated in aneurysmal bone cyst induces matrix metalloproteinase production via activation of NF-kappaB.
Ye Y, Pringle LM, Lau AW, Riquelme DN, Wang H, Jiang T, Lev D, Welman A, Blobel GA, Oliveira AM, Chou MM
Oncogene 2010 Jun 24;29(25):3619-29
Oncogene 2010 Jun 24;29(25):3619-29
Fibroblast-conditioned media promote human sarcoma cell invasion.
Bittner JG 4th, Wilson M, Shah MB, Albo D, Feig BW, Wang TN
Surgery 2009 Jan;145(1):42-7
Surgery 2009 Jan;145(1):42-7
Development and validation of sandwich ELISA microarrays with minimal assay interference.
Gonzalez RM, Seurynck-Servoss SL, Crowley SA, Brown M, Omenn GS, Hayes DF, Zangar RC
Journal of proteome research 2008 Jun;7(6):2406-14
Journal of proteome research 2008 Jun;7(6):2406-14
Molecular profiling of cervical cancer progression.
Hagemann T, Bozanovic T, Hooper S, Ljubic A, Slettenaar VI, Wilson JL, Singh N, Gayther SA, Shepherd JH, Van Trappen PO
British journal of cancer 2007 Jan 29;96(2):321-8
British journal of cancer 2007 Jan 29;96(2):321-8
Differential proteolytic enzyme activity in eosinophilic and neutrophilic asthma.
Simpson JL, Scott RJ, Boyle MJ, Gibson PG
American journal of respiratory and critical care medicine 2005 Sep 1;172(5):559-65
American journal of respiratory and critical care medicine 2005 Sep 1;172(5):559-65
Differential expression and activity of matrix metalloproteinases during flow-modulated vein graft remodeling.
Berceli SA, Jiang Z, Klingman NV, Pfahnl CL, Abouhamze ZS, Frase CD, Schultz GS, Ozaki CK
Journal of vascular surgery 2004 May;39(5):1084-90
Journal of vascular surgery 2004 May;39(5):1084-90
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- MMP-9 in MCF-7 Human Cell Line. MMP-9 was detected in immersion fixed MCF-7 human breast cancer cell line using Human MMP-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF911) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- MMP-9 in NS0 Mouse Cell Line. MMP-9 was detected in immersion fixed NS0 mouse myeloma cell line transfected with MMP-9 using 5 µg/mL Human MMP-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF911) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- MMP-9 in Human Ovarian Cancer Tissue. MMP-9 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Human MMP-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF911) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- MMP-9 in Human Ovarian Cancer Tissue. MMP-9 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Human MMP-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF911) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
