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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [15]
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- Western blot [1]
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- Product number
- MAB911 - Provider product page
- Provider
- R&D Systems
- Product name
- Human MMP-9 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human MMP-9 in Western blots. In Western blots, reactivity with the pro (92 kDa), active (82 kDa), and C-terminal truncated (65 kDa) forms of recombinant human (rh) MMP-9 is observed. Also in Western blots, 20% cross-reactivity with rhMMP-2, 5% cross-reactivity with rhMMP-1, and no cross-reactivity with rhMMP-3, -7, -8, -10, -12, or -13 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 4H3
- Vial size
- 200 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Diverse matrix metalloproteinase functions regulate cancer amoeboid migration.
The DSCs-expressed CD82 controls the invasiveness of trophoblast cells via integrinbeta1/MAPK/MAPK3/1 signaling pathway in human first-trimester pregnancy.
High matrix metalloproteinase production correlates with immune activation and leukocyte migration in leprosy reactional lesions.
Matrix metalloproteinase activity in pediatric acute lung injury.
Matrix metalloproteinase activity in pediatric acute lung injury.
Long-term weight loss decreases the nontraditional cardiovascular risk factors interleukin-18 and matrix metalloproteinase-9 in obese subjects.
Mediators of glioblastoma resistance and invasion during antivascular endothelial growth factor therapy.
Expression pattern and circulating levels of endostatin in patients with pancreas cancer.
Neutrophil gelatinase-associated lipocalin is expressed in osteoarthritis and forms a complex with matrix metalloproteinase 9.
IL-21 is highly produced in Helicobacter pylori-infected gastric mucosa and promotes gelatinases synthesis.
IL-21 is highly produced in Helicobacter pylori-infected gastric mucosa and promotes gelatinases synthesis.
Hypoxia-induced mediators of stem/progenitor cell trafficking are increased in children with hemangioma.
Role of platelet-derived growth factor and transforming growth factor beta1 the in the regulation of metalloproteinase expressions.
Control of matrix metalloproteinase production in human intestinal fibroblasts by interleukin 21.
Neutrophil-derived metalloproteinase-9 predicts healing quality after sinus surgery.
Orgaz JL, Pandya P, Dalmeida R, Karagiannis P, Sanchez-Laorden B, Viros A, Albrengues J, Nestle FO, Ridley AJ, Gaggioli C, Marais R, Karagiannis SN, Sanz-Moreno V
Nature communications 2014 Jun 25;5:4255
Nature communications 2014 Jun 25;5:4255
The DSCs-expressed CD82 controls the invasiveness of trophoblast cells via integrinbeta1/MAPK/MAPK3/1 signaling pathway in human first-trimester pregnancy.
Li MQ, Hou XF, Shao J, Tang CL, Li DJ
Biology of reproduction 2010 May;82(5):968-79
Biology of reproduction 2010 May;82(5):968-79
High matrix metalloproteinase production correlates with immune activation and leukocyte migration in leprosy reactional lesions.
Teles RM, Teles RB, Amadeu TP, Moura DF, Mendonça-Lima L, Ferreira H, Santos IM, Nery JA, Sarno EN, Sampaio EP
Infection and immunity 2010 Mar;78(3):1012-21
Infection and immunity 2010 Mar;78(3):1012-21
Matrix metalloproteinase activity in pediatric acute lung injury.
Kong MY, Gaggar A, Li Y, Winkler M, Blalock JE, Clancy JP
International journal of medical sciences 2009;6(1):9-17
International journal of medical sciences 2009;6(1):9-17
Matrix metalloproteinase activity in pediatric acute lung injury.
Kong MY, Gaggar A, Li Y, Winkler M, Blalock JE, Clancy JP
International journal of medical sciences 2009;6(1):9-17
International journal of medical sciences 2009;6(1):9-17
Long-term weight loss decreases the nontraditional cardiovascular risk factors interleukin-18 and matrix metalloproteinase-9 in obese subjects.
Madsen EL, Bruun JM, Skogstrand K, Hougaard DM, Christiansen T, Richelsen B
Metabolism: clinical and experimental 2009 Jul;58(7):946-53
Metabolism: clinical and experimental 2009 Jul;58(7):946-53
Mediators of glioblastoma resistance and invasion during antivascular endothelial growth factor therapy.
Lucio-Eterovic AK, Piao Y, de Groot JF
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Jul 15;15(14):4589-99
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Jul 15;15(14):4589-99
Expression pattern and circulating levels of endostatin in patients with pancreas cancer.
Ohlund D, Ardnor B, Oman M, Naredi P, Sund M
International journal of cancer 2008 Jun 15;122(12):2805-10
International journal of cancer 2008 Jun 15;122(12):2805-10
Neutrophil gelatinase-associated lipocalin is expressed in osteoarthritis and forms a complex with matrix metalloproteinase 9.
Gupta K, Shukla M, Cowland JB, Malemud CJ, Haqqi TM
Arthritis and rheumatism 2007 Oct;56(10):3326-35
Arthritis and rheumatism 2007 Oct;56(10):3326-35
IL-21 is highly produced in Helicobacter pylori-infected gastric mucosa and promotes gelatinases synthesis.
Caruso R, Fina D, Peluso I, Fantini MC, Tosti C, Del Vecchio Blanco G, Paoluzi OA, Caprioli F, Andrei F, Stolfi C, Romano M, Ricci V, MacDonald TT, Pallone F, Monteleone G
Journal of immunology (Baltimore, Md. : 1950) 2007 May 1;178(9):5957-65
Journal of immunology (Baltimore, Md. : 1950) 2007 May 1;178(9):5957-65
IL-21 is highly produced in Helicobacter pylori-infected gastric mucosa and promotes gelatinases synthesis.
Caruso R, Fina D, Peluso I, Fantini MC, Tosti C, Del Vecchio Blanco G, Paoluzi OA, Caprioli F, Andrei F, Stolfi C, Romano M, Ricci V, MacDonald TT, Pallone F, Monteleone G
Journal of immunology (Baltimore, Md. : 1950) 2007 May 1;178(9):5957-65
Journal of immunology (Baltimore, Md. : 1950) 2007 May 1;178(9):5957-65
Hypoxia-induced mediators of stem/progenitor cell trafficking are increased in children with hemangioma.
Kleinman ME, Greives MR, Churgin SS, Blechman KM, Chang EI, Ceradini DJ, Tepper OM, Gurtner GC
Arteriosclerosis, thrombosis, and vascular biology 2007 Dec;27(12):2664-70
Arteriosclerosis, thrombosis, and vascular biology 2007 Dec;27(12):2664-70
Role of platelet-derived growth factor and transforming growth factor beta1 the in the regulation of metalloproteinase expressions.
Borrelli V, di Marzo L, Sapienza P, Colasanti M, Moroni E, Cavallaro A
Surgery 2006 Sep;140(3):454-63
Surgery 2006 Sep;140(3):454-63
Control of matrix metalloproteinase production in human intestinal fibroblasts by interleukin 21.
Monteleone G, Caruso R, Fina D, Peluso I, Gioia V, Stolfi C, Fantini MC, Caprioli F, Tersigni R, Alessandroni L, MacDonald TT, Pallone F
Gut 2006 Dec;55(12):1774-80
Gut 2006 Dec;55(12):1774-80
Neutrophil-derived metalloproteinase-9 predicts healing quality after sinus surgery.
Watelet JB, Demetter P, Claeys C, Van Cauwenberge P, Cuvelier C, Bachert C
The Laryngoscope 2005 Jan;115(1):56-61
The Laryngoscope 2005 Jan;115(1):56-61
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human MMP-9 by Western Blot. Western blot shows lysates of U937 human histiocytic lymphoma cell line untreated (-) or treated (+) with 5 ng/mL PMA for 24 hours. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human MMP-9 Monoclonal Antibody (Catalog # MAB911) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MMP-9 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
