PA5-116985

antibody from Invitrogen Antibodies

Targeting: H3C2 H3/l, H3FL, HIST1H3B

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-116985

Provider

Invitrogen Antibodies

Product name

H3R8me2a Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Positive Samples: HeLa, NIH/3T3

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.37 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of H3R8me2a using H3R8me2a Polyclonal Antibody (Product # PA5-116985) at a 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis of H3R8me2a in 293T cells using H3R8me2a Polyclonal Antibody (Product # PA5-116985) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of H3R8me2a in human colon tissue using H3R8me2a Polyclonal Antibody (Product # PA5-116985) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of H3R8me2a in Rat testis tissue using H3R8me2a Polyclonal Antibody (Product # PA5-116985) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of H3R8me2a in Mouse testis tissue using H3R8me2a Polyclonal Antibody (Product # PA5-116985) at a dilution of 1:100.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Dot-blot analysis of all sorts of methylation peptides using H3R8me2a Polyclonal Antibody (Product # PA5-116985).