MA5-14029

antibody from Invitrogen Antibodies

Targeting: VWF F8VWF

Provider product page for MA5-14029

Western blot

Antibody data

Antibody Data
Antigen structure
References [28]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [3]
Flow cytometry [1]
Other assay [5]

Submit

Product number: MA5-14029 - Provider product page
Provider: Invitrogen Antibodies
Product name: VWF Monoclonal Antibody (F8/86)
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: MA5-14029 targets von Willebrand Factor in IHC (P) applications and shows reactivity with Human samples.
Antibody clone number: F8/86
Concentration: 0.2 mg/mL

VWF protein structure - MA5-14029 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Formalin-fixed, paraffin-embedded human tonsil stained with Factor VIII antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of endothelial cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of von Willebrand Factor (F8/86) showing staining in the endothelial cells of paraffin-embedded human colon tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a von Willebrand Factor Antibody (F8/86) Mouse Monoclonal Antibody (Product # MA5-14029) diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of von Willebrand Factor (F8/86) showing staining in the endothelial cells of paraffin-embedded mouse colon tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a von Willebrand Factor Antibody (F8/86) Mouse Monoclonal Antibody (Product # MA5-14029) diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 7 PRCP did not show overlap with vWF in stimulated HUVEC and HAoEC. Double staining of PRCP and VWF in control, LPS-, TNFalpha-, and IL-1beta-stimulated HUVEC. Cells were incubated for 16 h with the different stimuli, fixed with 4% PFA, permeabilized with 0.1% Triton X-100 and stained for PRCP (green) and vWF (red). Representative images of two independent experiments. (bar = 20 um).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: FIGURE 3 High VWF content and aberrant WPB distribution in CCM1 -/- BOECs. (A) Immunofluorescent staining demonstrated high-level VWF expression (green) in clonally expanded CCM1 -/- BOECs. DAPI (blue) was used as nuclear counterstain. Confocal images were acquired using a 63x (NA 1.4) oil objective (left) and at higher magnification shown as maximum intensity projection of image stacks (0.2 um z planes). Scale bars indicate 20 um. (B) Absolute amount of secreted VWF from CCM1 +/- and CCM1 -/- BOECs as quantified by ELISA. Data are presented as single data points with the mean ( n = 3). (C) Analysis strategy of high-content imaging for untreated and stimulated BOECs. As basis for quantification, a sliding parabola function was applied for contrast enhancement of the VWF signal (green). Cell nuclei were segmented by their DAPI signal (blue) and the surrounding cytosolic area and perinuclear region were detected. VWF-positive granules in the cytosol (I) and the perinuclear region (II) were quantified. Scale bar indicates 30 um. (D) WPBs in the perinuclear region were quantified as shown in untreated CCM1 +/- and CCM1 -/- BOECs. Data are presented as single data points with the mean. Two-way ANOVA with Sidak correction for multiple comparisons was used for statistical analysis. p < 0.05. All experiments were performed in triplicates and four biological replicates. (E) Representative images of WPBs in the perinuclear region in untreated CCM1 +/- (left) and CCM1 -/- BOECs (right)

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: FIGURE 4 High-level VWF expression is a common feature of CCM disease. (A) Strong immunopositivity for VWF (shown in green) was also found in clonally expanded CCM1 -/- CI-huVECs. DAPI (blue) was used as nuclear counterstain. Confocal images were acquired using a 10x (NA 0.45) objective. Scale bar indicates 50 um. (B) Immunohistochemistry demonstrated medium to strong VWF staining intensities (SI) in CCM tissue samples of hereditary cases (I-III). IV = normal brain. Representative images are shown. Open arrowhead = [SI] 4, black asterisk = [SI] 3, filled arrowhead = [SI] 2, open asterisk = [SI] 1, arrow = [SI] 0. Scale bars indicate 50 um. The graph displays the staining intensity of normal brain vessels in the vicinity of CCMs ( n = 13) and of cavernous vessel endothelia in CCM1 ( n = 11), CCM2 ( n = 3), and CCM3 ( n = 1) probands. Bars indicate the median.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Effect of the selected extracts on ECFC proliferation and differentiation. ( A ) Representative images of Ki67 and vWF staining of ECFCs treated 48 h with the ethanolic extracts of mango (4.99 mug/mL) and olive (46.67 mug/mL) leaves, and red grape pomace (11.67 mug/mL). ( B ) Box and whisker diagrams of the effect of the selected extracts on the proliferation and differentiation of ECFCs after 48 h of treatment. * p -value: * p < 0.05; ** p < 0.01; *** p < 0.001.